Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

Changes in trehalose content and trehalase activity during spore germination in fission yeast,... 203 129 129 1 1 Hirokazu Inoue Chikashi Shimoda Department of biology, Faculty of Science Osaka City University Sumiyoshi-ku 558 Osaka Japan Department of Tumor Viruses, Research Institute for Microbial Diseases Osaka University Yamada-kami 565 Suita, Osaka Japan Abstract Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

Archives of Microbiology , Volume 129 (1) – Mar 1, 1981

Loading next page...
 
/lp/springer-journals/changes-in-trehalose-content-and-trehalase-activity-during-spore-s0nKWO5Jc4

References (15)

Publisher
Springer Journals
Copyright
Copyright © 1981 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00417172
Publisher site
See Article on Publisher Site

Abstract

203 129 129 1 1 Hirokazu Inoue Chikashi Shimoda Department of biology, Faculty of Science Osaka City University Sumiyoshi-ku 558 Osaka Japan Department of Tumor Viruses, Research Institute for Microbial Diseases Osaka University Yamada-kami 565 Suita, Osaka Japan Abstract Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

Journal

Archives of MicrobiologySpringer Journals

Published: Mar 1, 1981

There are no references for this article.