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203 129 129 1 1 Hirokazu Inoue Chikashi Shimoda Department of biology, Faculty of Science Osaka City University Sumiyoshi-ku 558 Osaka Japan Department of Tumor Viruses, Research Institute for Microbial Diseases Osaka University Yamada-kami 565 Suita, Osaka Japan Abstract Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.
Archives of Microbiology – Springer Journals
Published: Mar 1, 1981
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