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Beta cells are important for islet innervation: evidence from purified rat islet-cell grafts

Beta cells are important for islet innervation: evidence from purified rat islet-cell grafts 125 39 39 1 1 U. Myrsén B. Keymeulen D. G. Pipeleers F. Sundler Department of Medical Cell Research University of Lund Lund Sweden Department of Metabolism and Endocrinology Free University of Brussels Brussels Belgium Summary Pancreatic islets receive an extensive and complex innervation that includes sympathetic, nor-adrenergic nerve fibres also storing neuropeptide Y. Islets transplanted to the kidney capsule become progressively reinnervated, mainly by sympathetic fibres and to a lesser extent by parasympathetic and sensory fibres. The density of nerve fibres in the islet grafts is often higher than in the graft-bearing organ, suggesting that the grafted islets contain factors that promote ingrowth of nerve fibres. To find out if beta cells are of any importance for attracting nerve fibres, purified preparations of rat islet beta and non-beta cells were transplanted to the kidneys of nude mice. Some of the mice were rendered diabetic by alloxan injection before transplantation. Immunocytochemical analysis revealed that the beta-cell grafts became richly re-innervated by noradrenergic (tyrosine hydroxylase-containing) nerve fibres, also storing neuropeptide Y. Non-beta islet-cell grafts were virtually devoid of demonstrable nerve fibres. There was no discernible difference in the reinnervation pattern between diabetic and non-diabetic mice. The findings indicate that factors mediating islet neurotrophism are produced by the beta cells http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetologia Springer Journals

Beta cells are important for islet innervation: evidence from purified rat islet-cell grafts

Myrsén, U.; Keymeulen, B.; Pipeleers, D.; Sundler, F.
Diabetologia , Volume 39 (1) – Jan 1, 1996
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References (36)

Publisher
Springer Journals
Copyright
Copyright © 1996 by Springer-Verlag
Subject
Medicine & Public Health; Human Physiology; Internal Medicine; Metabolic Diseases
ISSN
0012-186X
eISSN
1432-0428
DOI
10.1007/BF00400413
Publisher site
See Article on Publisher Site

Abstract

125 39 39 1 1 U. Myrsén B. Keymeulen D. G. Pipeleers F. Sundler Department of Medical Cell Research University of Lund Lund Sweden Department of Metabolism and Endocrinology Free University of Brussels Brussels Belgium Summary Pancreatic islets receive an extensive and complex innervation that includes sympathetic, nor-adrenergic nerve fibres also storing neuropeptide Y. Islets transplanted to the kidney capsule become progressively reinnervated, mainly by sympathetic fibres and to a lesser extent by parasympathetic and sensory fibres. The density of nerve fibres in the islet grafts is often higher than in the graft-bearing organ, suggesting that the grafted islets contain factors that promote ingrowth of nerve fibres. To find out if beta cells are of any importance for attracting nerve fibres, purified preparations of rat islet beta and non-beta cells were transplanted to the kidneys of nude mice. Some of the mice were rendered diabetic by alloxan injection before transplantation. Immunocytochemical analysis revealed that the beta-cell grafts became richly re-innervated by noradrenergic (tyrosine hydroxylase-containing) nerve fibres, also storing neuropeptide Y. Non-beta islet-cell grafts were virtually devoid of demonstrable nerve fibres. There was no discernible difference in the reinnervation pattern between diabetic and non-diabetic mice. The findings indicate that factors mediating islet neurotrophism are produced by the beta cells

Journal

Diabetologia – Springer Journals

Published: Jan 1, 1996

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