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Baseline and separation lines Part I: Where is the baseline? R. Kaiser i3adische Anilin- & Soda-Fabrik AG, Ammoniaklaboratorium, 19-67 Ludwigshafen Introductory remark: In each chromatographic laboratory, chromatograms have to be evaluated. No matter whether this is done with a rule and pencil, with a planirnete With scissors and scales, with a simple or a complicated integrato or even with an on-line computer: The application engineer is always confronted with the question where to locate the true baseline, i.e. where is the baseline, and where the separation lines. Much thought and experimental work has already been dedicated to this Problem, but still the opinions differ on this matter. In any case, the following statements will probably be correct: There are no truly straight baselines. A Very slight error in locating the baseline has a very large effect on the final result and can thus cause gross errors in the quantitative evaluation of chromatograms. In most cases, trace analyses are systematically evaluated in a wrong way, because the rules for locating baseline and separation lines are not observed. In most cases, electronic integrators systematically place Wrongly the baseline and separation lines, to a much larger extent than this is the
Chromatographia – Springer Journals
Published: Sep 1, 1969
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