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Huitao Liu, Ketai Wang, Xingguo Chen, Zhide Hu (2000)
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Electrophoretic behavior study and determination of some active components in Chinese medicinal preparations by capillary electrophoresis.The Analyst, 125 6
Orthogonal design has been used for optimization of the separation and determination of two active components (pair of isomers) in a traditional Chinese medicinal preparation by capillary electrophoresis. The concentration of borate and SDS, the organic modifier content and buffer pH were selected as variable parameters. Their different effects on peak resolution were studied by the design method. Optimized separation conditions were obtained and successfully applied for the first time to the separation and determination of imperatorin and isoimperatorin inYuanhu Zhiteng tablets within 7 min in a pH 8 buffer system: 25 mM borate, 15 mM SDS and 5% acetonitrile, detection at 214 nm. In addition, application of an artificial neural network with “4-7-1” structure to the prediction of peak resolution of the two active components given by uniform design was also examined and the predicted results were in good agreement with experimental values.
Chromatographia – Springer Journals
Published: Aug 28, 2006
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