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Application of experimental design and artificial neural networks to separation and determination of active components in traditional Chinese medicinal preparations by capillary electrophoresis

Application of experimental design and artificial neural networks to separation and determination... Orthogonal design has been used for optimization of the separation and determination of two active components (pair of isomers) in a traditional Chinese medicinal preparation by capillary electrophoresis. The concentration of borate and SDS, the organic modifier content and buffer pH were selected as variable parameters. Their different effects on peak resolution were studied by the design method. Optimized separation conditions were obtained and successfully applied for the first time to the separation and determination of imperatorin and isoimperatorin inYuanhu Zhiteng tablets within 7 min in a pH 8 buffer system: 25 mM borate, 15 mM SDS and 5% acetonitrile, detection at 214 nm. In addition, application of an artificial neural network with “4-7-1” structure to the prediction of peak resolution of the two active components given by uniform design was also examined and the predicted results were in good agreement with experimental values. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Application of experimental design and artificial neural networks to separation and determination of active components in traditional Chinese medicinal preparations by capillary electrophoresis

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References (16)

Publisher
Springer Journals
Copyright
Copyright © 2002 by Friedr. Vieweg & Sohn Verlagsgesellschaft mbH
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1007/BF02492904
Publisher site
See Article on Publisher Site

Abstract

Orthogonal design has been used for optimization of the separation and determination of two active components (pair of isomers) in a traditional Chinese medicinal preparation by capillary electrophoresis. The concentration of borate and SDS, the organic modifier content and buffer pH were selected as variable parameters. Their different effects on peak resolution were studied by the design method. Optimized separation conditions were obtained and successfully applied for the first time to the separation and determination of imperatorin and isoimperatorin inYuanhu Zhiteng tablets within 7 min in a pH 8 buffer system: 25 mM borate, 15 mM SDS and 5% acetonitrile, detection at 214 nm. In addition, application of an artificial neural network with “4-7-1” structure to the prediction of peak resolution of the two active components given by uniform design was also examined and the predicted results were in good agreement with experimental values.

Journal

ChromatographiaSpringer Journals

Published: Aug 28, 2006

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