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Analysis of vasopressin-induced Ca 2+ increase in rat hepatocytes

Analysis of vasopressin-induced Ca 2+ increase in rat hepatocytes To analyze vasopressin-induced Ca 2+ increase in liver cells, rat hepatocytes were isolated and attached to collagen-coated cover slips. Usipg fura-2, a Ca 2+ -sensing dye, changes in intracellular Ca 2+ concentration by vasopressin were monitored. Results in this communication suggested that vasopressin-induced Ca 2+ increase were composed of both Ca 2+ release from internal Ca 2+ stores and influx from the plasma membrane. The Ca 2+ influx consisted of two distinguishable components. One was dependent on the presence of vasopressin and the other was not. SK&F96365 blocked vasopressin-induced Ca 2+ influx in a dose-dependent manner. Vasopressin-induced Ca 2+ release from internal stores diminished in a primary culture of hepatocytes according to the culture time. However, changes in vasopressin-induced Ca 2+ influx across the plasma membrane differed from changes in the Ca 2+ release from internal stores, suggesting two separate signalings from receptor activation to internal stores and to the plasma membrane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Pharmacal Research Springer Journals

Analysis of vasopressin-induced Ca 2+ increase in rat hepatocytes

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References (20)

Publisher
Springer Journals
Copyright
Copyright © 2003 by The Pharmaceutical Society of Korea
Subject
Pharmacy; Pharmacy; Pharmacology/Toxicology
ISSN
0253-6269
eISSN
1976-3786
DOI
10.1007/BF03179934
Publisher site
See Article on Publisher Site

Abstract

To analyze vasopressin-induced Ca 2+ increase in liver cells, rat hepatocytes were isolated and attached to collagen-coated cover slips. Usipg fura-2, a Ca 2+ -sensing dye, changes in intracellular Ca 2+ concentration by vasopressin were monitored. Results in this communication suggested that vasopressin-induced Ca 2+ increase were composed of both Ca 2+ release from internal Ca 2+ stores and influx from the plasma membrane. The Ca 2+ influx consisted of two distinguishable components. One was dependent on the presence of vasopressin and the other was not. SK&F96365 blocked vasopressin-induced Ca 2+ influx in a dose-dependent manner. Vasopressin-induced Ca 2+ release from internal stores diminished in a primary culture of hepatocytes according to the culture time. However, changes in vasopressin-induced Ca 2+ influx across the plasma membrane differed from changes in the Ca 2+ release from internal stores, suggesting two separate signalings from receptor activation to internal stores and to the plasma membrane.

Journal

Archives of Pharmacal ResearchSpringer Journals

Published: Jan 1, 2003

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