122 89 89 7 8 J. W. van Ooijen J. M. Sandbrink M. Vrielink R. Verkerk P. Zabel P. Lindhout Centre for Plant Breeding and Reproduction Research (CPRO-DLO) P.O. Box 16 6700 AA Wageningen The Netherlands Department of Molecular Biology Agricultural University Dreijenlaan 3 6703 HA Wageningen The Netherlands Department of Plant Breeding Agricultural University Lawickse Allee 166 6709 DB Wageningen The Netherlands Abstract In order to map genes determining resistance to bacterial canker in tomato, backcrosses were made between a resistant and a susceptible Lycopersicon peruvianum accession. The linkage study with RFLP markers yielded a genetic map of L. Peruvianum . This map was compared to that derived from a L. esculentum x L. pennellii F 2 population, based on 70 shared RFLP markers. The maps showed a good resemblance in both the order of markers and the length of the chromosomes, with the exception of just one relocated marker on chromosome 9. Because backcrosses were made with the F 1 , either as the pollen parent or as the pistil parent, linkage maps from male and female meioses could be estimated. It was concluded that recombination at male meiosis was reduced, and that gametophytic selection for parental genotypes at more than one locus per chromosome might be partly responsible for the reduction of the estimated male map length.
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