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An improved enzymatic reagent for visualization of phosphate esters in TLC

An improved enzymatic reagent for visualization of phosphate esters in TLC A sensitive spray reagent for visualization in TLC of phosphate esters is obtained by deglobulinization and purification of horse serum. The spots, produced in the areas where the phosphate esters inhibit the choline esterase of the serum, are clear and sharp-edged against a dark background. The reagent can be prepared in bulk quantities, is not expensive and can be stored at 4°C without deterioration for a long time (6–12 months). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

An improved enzymatic reagent for visualization of phosphate esters in TLC

Chromatographia , Volume 9 (12) – Dec 1, 1976

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References (1)

Publisher
Springer Journals
Copyright
Copyright © 1976 by Friedr. Vieweg & Sohn, Verlagschesellschaft mbH
Subject
Chemistry; Analytical Chemistry; Organic Chemistry; Biochemistry, general; Plant Sciences; Pharmacy; Measurement Science, Instrumentation
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1007/BF02290535
Publisher site
See Article on Publisher Site

Abstract

A sensitive spray reagent for visualization in TLC of phosphate esters is obtained by deglobulinization and purification of horse serum. The spots, produced in the areas where the phosphate esters inhibit the choline esterase of the serum, are clear and sharp-edged against a dark background. The reagent can be prepared in bulk quantities, is not expensive and can be stored at 4°C without deterioration for a long time (6–12 months).

Journal

ChromatographiaSpringer Journals

Published: Dec 1, 1976

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