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A PCR-based marker tightly linked to the nematode resistance gene, Mi , in tomato

A PCR-based marker tightly linked to the nematode resistance gene, Mi , in tomato 122 87 87 7 7 V. M. Williamson J. -Y. Ho F. F. Wu N. Miller I. Kaloshian Department of Nematology University of California 95616 Davis CA USA Department of Medical Genetics University of British Columbia V6T 1Z3 Vancouver B.C. Canada Abstract A PCR-based codominant marker has been developed which is tightly linked to Mi , a dominant genetic locus in tomato that confers resistance to several species of root-knot nematode. DNA from tomato lines differing in nematode resistance was screened for random amplified polymorphic DNA markers linked to Mi using decamer primers. Several markers were identified. One amplified product, REX-1, obtained using a pair of decamer primers, was present as a dominant marker in all nematode-resistant tomato lines tested. REX-1 was cloned and the DNA sequences of its ends were determined and used to develop 20-mer primers. PCR amplification with the 20-mer primers produced a single amplified band in both susceptible and resistant tomato lines. The amplified bands from susceptible and resistant lines were distinguishable after cleavage with the restriction enzyme Taq I. The linkage of REX-1 to Mi was verified in an F 2 population. This marker is more tightly linked to Mi than is Aps-1 , the currently-used isozyme marker, and allows screening of germplasm where the linkage between Mi and Aps-1 has been lost. Homozygous and heterozygous individuals can be distinguished and the procedure can be used for rapid, routine screening. The strategy used to obtain REX-1 is applicable to obtaining tightly-linked markers to other genetic loci. Such markers would allow rapid, concurrent screening for the segregation of several loci of interest. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

A PCR-based marker tightly linked to the nematode resistance gene, Mi , in tomato

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References (36)

Publisher
Springer Journals
Copyright
Copyright © 1994 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00221126
pmid
24190460
Publisher site
See Article on Publisher Site

Abstract

122 87 87 7 7 V. M. Williamson J. -Y. Ho F. F. Wu N. Miller I. Kaloshian Department of Nematology University of California 95616 Davis CA USA Department of Medical Genetics University of British Columbia V6T 1Z3 Vancouver B.C. Canada Abstract A PCR-based codominant marker has been developed which is tightly linked to Mi , a dominant genetic locus in tomato that confers resistance to several species of root-knot nematode. DNA from tomato lines differing in nematode resistance was screened for random amplified polymorphic DNA markers linked to Mi using decamer primers. Several markers were identified. One amplified product, REX-1, obtained using a pair of decamer primers, was present as a dominant marker in all nematode-resistant tomato lines tested. REX-1 was cloned and the DNA sequences of its ends were determined and used to develop 20-mer primers. PCR amplification with the 20-mer primers produced a single amplified band in both susceptible and resistant tomato lines. The amplified bands from susceptible and resistant lines were distinguishable after cleavage with the restriction enzyme Taq I. The linkage of REX-1 to Mi was verified in an F 2 population. This marker is more tightly linked to Mi than is Aps-1 , the currently-used isozyme marker, and allows screening of germplasm where the linkage between Mi and Aps-1 has been lost. Homozygous and heterozygous individuals can be distinguished and the procedure can be used for rapid, routine screening. The strategy used to obtain REX-1 is applicable to obtaining tightly-linked markers to other genetic loci. Such markers would allow rapid, concurrent screening for the segregation of several loci of interest.

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Feb 1, 1994

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