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A high-performance liquid chromatographic method for the analysis of adenosine and some metabolites in the brain tissue of rats

A high-performance liquid chromatographic method for the analysis of adenosine and some... An analytical procedure has been developed for the assay of adenosine and some metabolites in brain tissue extracts of rats. This paper reports the extraction method, the technique adopted to avoid enzymatic transformations and the chromatographic conditions for the identification and quantitation of these nucleosides and bases. Peaks in the chromatograms of brain tissue extracts were identified by retention times, absorbance ratios of reference compounds and by enzymatic peak-shift. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

A high-performance liquid chromatographic method for the analysis of adenosine and some metabolites in the brain tissue of rats

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References (19)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Friedr. Vieweg & Sohn Verlagsgesellschaft mbH
Subject
Chemistry; Analytical Chemistry; Organic Chemistry; Biochemistry, general; Plant Sciences; Pharmacy; Measurement Science, Instrumentation
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1007/BF02269864
Publisher site
See Article on Publisher Site

Abstract

An analytical procedure has been developed for the assay of adenosine and some metabolites in brain tissue extracts of rats. This paper reports the extraction method, the technique adopted to avoid enzymatic transformations and the chromatographic conditions for the identification and quantitation of these nucleosides and bases. Peaks in the chromatograms of brain tissue extracts were identified by retention times, absorbance ratios of reference compounds and by enzymatic peak-shift.

Journal

ChromatographiaSpringer Journals

Published: Dec 1, 1992

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