Cdc42 desensitizes the contractile apparatus of isolated resistance arteries (RA) to ~ a 2 +via p21-activated kinase (PAK) SS Bolz, AV Somlyo, AP Somlyo, E Manser, U Pohl Physiologisches Institut der LMU RhoA, Rac and Cdc42 regulate diverse smooth muscle cell functions by modulating myosin light chain phosphorylation. While RhoA and Rac are known to regulate RA tone by this mechanism, little is known about the involvement of Cdc42. Hamster RA (diameter l97IlOym) were transfected for 19-22h with plasmids codin for GFP (n=6), Cdc42 (n=7), constitutively active PAK (PAKL 07F, n=5), or Cdc42 plus a PAK-inhibiting peptide ( ~ d c 4 2 + ~ ~ ~ r e ~ 8 3 ,Subsequentl n=4). the Ca2+ sensitivity (relationship between diameter and [Caxili, raised by increasing [ca2+leX around depolarized RA) and the myogenic response (MR, pressure step 45 to 110 mmHg) were determined. Cdc42 and P A K L ~ O ~ significantly reduced resting tone of RA F compared to GFP controls, significantly attenuated constrictions elicited by increasing [ca2+li and abolished MRs (Cdc42 2I2%, P A K L ~ ~ 1I2%, GFP 59I6% reversal of distension, RD). ~F Coexpression of P A K ' ~ S with Cdc42
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83rd Annual Meeting DPG. Abstracts pp S45-S64
Follow Pflügers Archiv European Journal of Physiologyl of Physiology
, Volume 447 (1)
Springer Journals – Mar 1, 2004
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