“Woah! It's like Spotify but for academic articles.”

Instant Access to Thousands of Journals for just $40/month

Get 2 Weeks Free

Role of H 2 O 2 in changing β-adrenoceptor and adenylyl cyclase in ischemia-reperfused hearts

Role of H 2 O 2 in changing β-adrenoceptor and adenylyl cyclase in ischemia-reperfused hearts In view of the accumulation of H 2 O 2 in the myocardium due to ischemia-reperfusion and changes in β-adrenoceptor mechanisms in the ischemic-reperfused heart, we investigated the effects of H 2 O 2 on the β-adrenoceptor, G-protein and adenylyl cyclase complex. Rat hearts were perfused with 1 mM H 2 O 2 for 10 min before isolating membranes for measuring the biochemical activities. The stimulation of adenylyl cyclase by different concentrations of isoproterenol was depressed upon perfusing hearts with H 2 O 2 . Both the affinity and density of β 1 -adrenoceptors as well as the density of the β 2 -adrenoceptors were decreased whereas the affinity of β 2 -adrenoceptors was increased by H 2 O 2 perfusion. Competition curves did not reveal any effect of H 2 O 2 on the proportion of coupled receptors in the high affinity state. The basal as well as forskolin-, NaF- and Gpp(NH)p-stimulated adenylyl cyclase activities were depressed by perfusing the heart with H 2 O 2 . Catalase alone or in combination with mannitol was able to significantly decrease the magnitude of alterations due to H 2 O 2 . The positive inotropic effect of 1 μM isoproterenol was markedly attenuated upon perfusing hearts with 200-500 μM H 2 O 2 for 10 min. These results suggest that H 2 O 2 may depress the β 1 -adrenoceptor, G s -proteins and catalytic subunit of the adenylyl cyclase enzyme and thus may play an important role in attenuating the β-adrenoceptor linked signal transduction due to ischemia-reperfusion injury. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Cellular Biochemistry Springer Journals
Loading next page...
 
/lp/springer-journal/role-of-h-2-o-2-in-changing-adrenoceptor-and-adenylyl-cyclase-in-uEU4Dw9ydN

You're reading a free preview. Subscribe to read the entire article.

And millions more from thousands of peer-reviewed journals, for just $40/month

Get 2 Weeks Free

To be the best researcher, you need access to the best research

  • With DeepDyve, you can stop worrying about how much articles cost, or if it's too much hassle to order — it's all at your fingertips. Your research is important and deserves the top content.
  • Read from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more.
  • All the latest content is available, no embargo periods.

Stop missing out on the latest updates in your field

  • We’ll send you automatic email updates on the keywords and journals you tell us are most important to you.
  • There is a lot of content out there, so we help you sift through it and stay organized.