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Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv

Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv 203 144 144 2 2 K. T. Hiriyanna T. Ramakrishnan Microbiology and Cell Biology Laboratory Indian Institute of Science 560012 Bangalore India ICMR Unit on Genetics and Cell Biology Indian Institute of science 560012 Bangalore India Department of Biology Johns Hopkins University 21218 Baltimore MD USA Abstract The DNA increment method, designed for measuring the increment in the amount of DNA after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (DNA) chain growth in Mycobacterium tuberculosis H37Rv growing in Youman and Karlson's medium at 37°C with a generation time of 24 h and also in relatively fast growing species like Mycobacterium smegmatis and Escherichia coli . From the results obtained, the time required for a DNA replication fork to traverse the chromosome from origin to terminus (C period) was calculated. The chain elongation rates of DNA of the three organisms was determined from the C period and the known genome sizes assuming that all these genomes have a single replication origin and bidirectional replication fork. The rate for M. tuberculosis was 3,200 nucleotides per min about 11 times slower than that of M. smegmatis and about 13–18 times slower than that of E. coli . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv

Archives of Microbiology , Volume 144 (2) – Mar 1, 1986

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References (16)

Publisher
Springer Journals
Copyright
Copyright © 1986 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00414718
Publisher site
See Article on Publisher Site

Abstract

203 144 144 2 2 K. T. Hiriyanna T. Ramakrishnan Microbiology and Cell Biology Laboratory Indian Institute of Science 560012 Bangalore India ICMR Unit on Genetics and Cell Biology Indian Institute of science 560012 Bangalore India Department of Biology Johns Hopkins University 21218 Baltimore MD USA Abstract The DNA increment method, designed for measuring the increment in the amount of DNA after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (DNA) chain growth in Mycobacterium tuberculosis H37Rv growing in Youman and Karlson's medium at 37°C with a generation time of 24 h and also in relatively fast growing species like Mycobacterium smegmatis and Escherichia coli . From the results obtained, the time required for a DNA replication fork to traverse the chromosome from origin to terminus (C period) was calculated. The chain elongation rates of DNA of the three organisms was determined from the C period and the known genome sizes assuming that all these genomes have a single replication origin and bidirectional replication fork. The rate for M. tuberculosis was 3,200 nucleotides per min about 11 times slower than that of M. smegmatis and about 13–18 times slower than that of E. coli .

Journal

Archives of MicrobiologySpringer Journals

Published: Mar 1, 1986

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