Subscribe to thousands of academic journals for just $40/month
Read and share the articles you need for your research, all in one place.

Long-Term Effect on EMT6 Tumors in Mice Induced by Combination of Laser Immunotherapy and Surgery

Integrative Cancer Therapies , Volume 10 (4): 368 – Dec 1, 2011


Sage Publications
Copyright © 2011 by SAGE Publications
Publisher site
See Article on Publisher Site

Preview Only

Expand Tray Hide Tray

Long-Term Effect on EMT6 Tumors in Mice Induced by Combination of Laser Immunotherapy and Surgery


Laser immunotherapy (LI) has been demonstrated to be a promising modality for cancer treatment. The present study was designed to further investigate the impact of LI combined with surgery. LI consists of a near-infrared laser, a light-absorbing dye (indocyanine green, ICG), and an immunostimulant (glycated chitosan, GC). ICG and GC were intratumorally injected, followed by laser irradiation. Female BALB/c mice bearing EMT6 tumor cells were divided into 4 groups: control, LI, LI followed by immediate surgery resection of residual tumor (LI + S0wk), and LI followed by surgical removal of residual tumor after 1 week (LI + S1wk). Successfully treated mice from all treatment groups were rechallenged twice with 105 and 5 × 105 EMT6 cells, respectively. The LI + S1wk group had the highest survival rate (72%) after 90 days, whereas the mice survival rates of the LI + S0wk, LI, and control groups were 50%, 46%, 0%, respectively. The median survival times of control, LI, LI + S0wk, and LI + S1wk groups were 32, 66, 74, and 90 days, respectively. Survival rates of the treated mice after the first and second tumor rechallenges, ranging from 73% to 95%, were not significantly different among the 4 groups (P > .05). The results show that LI is a useful tool for the treatment of tumor-bearing mice. Long-term antitumor effect can be induced by LI. They also indicate that combination of LI with surgery can further improve the therapeutic efficiency of LI.
Loading next page...

Preview Only. This article cannot be rented because we do not currently have permission from the publisher.