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THE ULTRACENTRIFUGE STUDY OF PLASTEIN

THE ULTRACENTRIFUGE STUDY OF PLASTEIN (Received for publication, March 10, 1947) Two samples of plastein, prepared by J. H. Northrop (1), were studied in the ultracentrifuge in an effort to determine the characteristics of the material. One sample was prepared from a hydrolyzed trypsin solution, the other from a hydrolyzed pepsin solution. Both samples were dissolved in phosphate buffer at a pH of 10.1 to a concentration of 1 per cent, the buffer being 0.032 ~ in NazHPO4 and 0.001 ~r in NaOH. The runs were made at about 10°C. for 45 minutes at a speed of 57,600 R.p.~r. in a centrifugal field of about 260,000 g. No sedimentation was observed during the interval which points to the fact that if any large particles were present their molecular weights are probably below 1000. This is in accord with the results obtained by Svedberg on plastein prepared by Folley (2). Under equivalent conditions, Svedberg was unable to demonstrate any sedimentation in the samples submitted to him. BIBLIOGRAPHY 1. Northrop, J. H., f. Gen. Physiol., 1947, 30, 377. 2. FoUey, S. J., Biochem. J., 1932, 26, 99. The Journal of General Physiology http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of General Physiology Rockefeller University Press

THE ULTRACENTRIFUGE STUDY OF PLASTEIN

The Journal of General Physiology , Volume 30 (5): 399 – May 1, 1947

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Publisher
Rockefeller University Press
Copyright
Copyright © 1947 by The Rockefeller University Press
ISSN
0022-1295
eISSN
1540-7748
DOI
10.1085/jgp.30.5.399
Publisher site
See Article on Publisher Site

Abstract

(Received for publication, March 10, 1947) Two samples of plastein, prepared by J. H. Northrop (1), were studied in the ultracentrifuge in an effort to determine the characteristics of the material. One sample was prepared from a hydrolyzed trypsin solution, the other from a hydrolyzed pepsin solution. Both samples were dissolved in phosphate buffer at a pH of 10.1 to a concentration of 1 per cent, the buffer being 0.032 ~ in NazHPO4 and 0.001 ~r in NaOH. The runs were made at about 10°C. for 45 minutes at a speed of 57,600 R.p.~r. in a centrifugal field of about 260,000 g. No sedimentation was observed during the interval which points to the fact that if any large particles were present their molecular weights are probably below 1000. This is in accord with the results obtained by Svedberg on plastein prepared by Folley (2). Under equivalent conditions, Svedberg was unable to demonstrate any sedimentation in the samples submitted to him. BIBLIOGRAPHY 1. Northrop, J. H., f. Gen. Physiol., 1947, 30, 377. 2. FoUey, S. J., Biochem. J., 1932, 26, 99. The Journal of General Physiology

Journal

The Journal of General PhysiologyRockefeller University Press

Published: May 1, 1947

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