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(Received for publication, March 10, 1947) Two samples of plastein, prepared by J. H. Northrop (1), were studied in the ultracentrifuge in an effort to determine the characteristics of the material. One sample was prepared from a hydrolyzed trypsin solution, the other from a hydrolyzed pepsin solution. Both samples were dissolved in phosphate buffer at a pH of 10.1 to a concentration of 1 per cent, the buffer being 0.032 ~ in NazHPO4 and 0.001 ~r in NaOH. The runs were made at about 10°C. for 45 minutes at a speed of 57,600 R.p.~r. in a centrifugal field of about 260,000 g. No sedimentation was observed during the interval which points to the fact that if any large particles were present their molecular weights are probably below 1000. This is in accord with the results obtained by Svedberg on plastein prepared by Folley (2). Under equivalent conditions, Svedberg was unable to demonstrate any sedimentation in the samples submitted to him. BIBLIOGRAPHY 1. Northrop, J. H., f. Gen. Physiol., 1947, 30, 377. 2. FoUey, S. J., Biochem. J., 1932, 26, 99. The Journal of General Physiology
The Journal of General Physiology – Rockefeller University Press
Published: May 1, 1947
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