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Synaptic vesicle structure

Synaptic vesicle structure <h2>Synaptic vesicle structure</h2> A synaptic vesicle, up close and personal. TAKAMORI/ELSEVIER The first molecular level model of an organelle is described by Shigeo Takamori, Reinhard Jahn (Max-Planck Institute, Göttingen, Germany), and colleagues. Proteomics, quantitative measurements, and molecular modeling give them a view of an average synaptic vesicle. The first surprise is that the vesicle is almost smothered in proteins. The group started by identifying 410 proteins associated with purified brain synaptic vesicles, but “if you want to put 400 proteins in the model it exceeds the amount of surface area available,” says Takamori. They suspect that many of the proteins are passengers on only a few vesicles, so instead they measured the copy number of the more abundant proteins. By putting just these 27 proteins in the model, they accounted for almost 70% by mass of the vesicles' protein complement. Even with these few proteins, “the vesicles are really protein dominated,” says Takamori. Transmembrane domains take up approximately 20% of the surface, with their attached globular domains shading far more. If each protein was surrounded by a monomeric collar of phospholipids, the majority of phospholipids would not be free. The amount of protein and lipid clustering is not http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

Synaptic vesicle structure

Wells, William A.
The Journal of Cell Biology , Volume 175 (5): 679 – Dec 4, 2006
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References (1)

  • S. Takamori, M. Holt, K. Stenius, E. Lemke, M. Grønborg, D. Riedel, H. Urlaub, S. Schenck, B. Brügger, P. Ringler, S. Müller, Burkhard Rammner, F. Gräter, J. Hub, B. Groot, G. Mieskes, Y. Moriyama, J. Klingauf, H. Grubmüller, J. Heuser, F. Wieland, R. Jahn (2006)

    Molecular Anatomy of a Trafficking Organelle

    Cell, 127

Publisher
Rockefeller University Press
Copyright
© 2006 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.1755rr5
Publisher site
See Article on Publisher Site

Abstract

<h2>Synaptic vesicle structure</h2> A synaptic vesicle, up close and personal. TAKAMORI/ELSEVIER The first molecular level model of an organelle is described by Shigeo Takamori, Reinhard Jahn (Max-Planck Institute, Göttingen, Germany), and colleagues. Proteomics, quantitative measurements, and molecular modeling give them a view of an average synaptic vesicle. The first surprise is that the vesicle is almost smothered in proteins. The group started by identifying 410 proteins associated with purified brain synaptic vesicles, but “if you want to put 400 proteins in the model it exceeds the amount of surface area available,” says Takamori. They suspect that many of the proteins are passengers on only a few vesicles, so instead they measured the copy number of the more abundant proteins. By putting just these 27 proteins in the model, they accounted for almost 70% by mass of the vesicles' protein complement. Even with these few proteins, “the vesicles are really protein dominated,” says Takamori. Transmembrane domains take up approximately 20% of the surface, with their attached globular domains shading far more. If each protein was surrounded by a monomeric collar of phospholipids, the majority of phospholipids would not be free. The amount of protein and lipid clustering is not

Journal

The Journal of Cell Biology – Rockefeller University Press

Published: Dec 4, 2006

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