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R. Sheinin, P. Darragh, M. Dubský (1978)
Some properties of chromatin synthesized by mouse-L-cells temperature-sensitive in DNA replication.The Journal of biological chemistry, 253 3
R. Hand (1975)
Regulation of DNA replication on subchromosomal units of mammalian cellsThe Journal of Cell Biology, 64
B. Blondel (1968)
Relation between nuclear fine structure and 3H-thymidine incorporation in a synchronous cell culture☆Experimental Cell Research, 53
E. Weibel, G. Kistler, W. Scherle (1966)
PRACTICAL STEREOLOGICAL METHODS FOR MORPHOMETRIC CYTOLOGYThe Journal of Cell Biology, 30
D. Comings (1967)
Sex chromatin, nuclear size and the cell cycle.Cytogenetics, 6 2
W. Sawicki, J. Rowiński, Ronald Swenson (1974)
Change of chromatin morphology during the cell cycle detected by means of automated image analysisJournal of Cellular Physiology, 84
D. Mazia (1963)
SYNTHETIC ACTIVITIES LEADING TO MITOSIS.Journal of cellular and comparative physiology, 62
F. Ruch, A. Rosselet (1970)
A cytochemical study of euchromatin and heterochromatin in roots of Rhoeo discolor.Experimental cell research, 62 1
D. Comings (1967)
The duration of replication of the inactive X chromosome in humans based on the persistence of the heterochromatic sex chromatin body during DNA synthesis.Cytogenetics, 6 1
Sheinin (1976)
Polyoma and cell DNA synthesis in mouse L cells temperature sensitive for the replication of cell DNAJournal of Virology, 17
H. Klinger, H. Schwarzacher, J. Weiss (1967)
DNA content and size of sex chromatin positive female nuclei during the cell cycle.Cytogenetics, 6 1
J. Lafontaine, A. Lord (1974)
An ultrastructural and radioautographic study of the evolution of the interphase nucleus in plant meristematic cells (Allium porrum).Journal of cell science, 14 2
R. Sheinin, P. Darragh, M. Dubský (1977)
Mitochondrial DNA synthesis in mouse L cells temperature sensitive in nuclear DNA replication.Canadian journal of biochemistry, 55 5
(1972)
Chromatin structure and the cell division cycle, actinomycin binding in synchronized HeLd cells
J. Finch, A. Klug (1976)
Solenoidal model for superstructure in chromatin.Proceedings of the National Academy of Sciences of the United States of America, 73 6
(1974)
Induction of chromosome condensation in interphase cells
P. Oudet, M. Gross-Bellard, P. Chambon (1975)
Electron microscopic and biochemical evidence that chromatin structure is a repeating unitCell, 4
(1971)
The ultrastructure of synchronized HeLd cells
K. Brasch, G. Setterfield, J. Neelin (1972)
Effects of sequential extraction of histone proteins on structural organization of avian erythrocyte and liver nuclei.Experimental cell research, 74 1
G. Milner (1969)
Nuclear morphology and the ultrastructural localization of deoxyribonucleic acid synthesis during interphase.Journal of cell science, 4 3
C. Nicolini, K. Ajiro, T. Borun, R. Baserga (1975)
Chromatin changes during the cell cycle of HeHa cells.The Journal of biological chemistry, 250 9
H. Crissman, P. Mullaney, J. Steinkamp (1975)
Methods and applications of flow systems for analysis and sorting of mammalian cells.Methods in cell biology, 9 0
(1975)
Eucaryotic chromosome replication
(1976)
Histone phosphorylation related to chromatin structure
K. Tokuyasu, S. Madden, L. Zeldis (1968)
FINE STRUCTURAL ALTERATIONS OF INTERPHASE NUCLEI OF LYMPHOCYTES STIMULATED TO GROWTH ACTIVITY IN VITROThe Journal of Cell Biology, 39
R. Johnson, A. Mullinger (1975)
The induction of DNA synthesis in the chick red cell nucleus in heterokaryons during the first cell cycle after fusion with HeLa cells.Journal of cell science, 18 3
F. Wunderlich, G. Herlan (1977)
Reversibly contractile nuclear matrix. Its isolation, structure, and compositionThe Journal of Cell Biology, 73
R. Martin, I. Radford, M. Pardee (1977)
Accumulation of short DNA fragments in hydroxyurea treated mouse L-cells.Biochemical and biophysical research communications, 74 1
R. Sheinin, S. Guttman (1977)
Semi-conservative and non-conservative replication of DNA in temperature-sensitive mouse L-cells.Biochimica et biophysica acta, 479 1
H. Crissman, P. Mullaney, J. Steinkamp (1975)
Chapter 12 Methods and Applications of Flow Systems for Analysis and Sorting of Mammalian Cells1Methods in Cell Biology, 9
Friedrich Back (1976)
The variable condition of euchromatin and heterochromatin.International review of cytology, 45
D. Comings (1968)
The rationale for an ordered arrangement of chromatin in the interphase nucleus.American journal of human genetics, 20 5
W. Nagl (1970)
The Mitotic and Endomitotic Nuclear Cycle in Allium Carinatum II. Relations Between DNA Replication and Chromatin StructureCaryologia, 23
L. Thompson, R. Mankovitz, R. Baker, J. Till, L. Siminovitch, G. Whitmore (1970)
Isolation of temperature-sensitive mutants of L-cells.Proceedings of the National Academy of Sciences of the United States of America, 66 2
N. Ringertz, J. Ericsson, O. Nilsson (1967)
Macronuclear chromatin structure in Euplotes.Experimental cell research, 48 1
S. Fakan, R. Hancock (1974)
Localization of newly-synthesized DNA in a mammalian cell as visualized by high resolution autoradiography.Experimental cell research, 83 1
A. Lord, J. Lafontaine (1976)
An ultrastructural and radioautographic study of the chromocentric interphase nucleus in plant meristematic cells (Raphanus sativus).Journal of cell science, 21 1
(1971)
Selective and non-selective isolation of temperature-sensitive mutants of mouse L-ceUs and their characterization
T. Pederson (1972)
Chromatin structure and the cell cycle.Proceedings of the National Academy of Sciences of the United States of America, 69 8
(1972)
The ordered replication of chromosomal DNA: a review and a proposal for its control
A. Olins, M. Senior, D. Olins (1976)
Ultrastructural features of chromatin nu bodiesThe Journal of Cell Biology, 68
R. Sheinin (1976)
Preliminary characterization of the temperature-sensitive defect in DNA replication in a mutant mouse L cellCell, 7
C. Geary (1979)
Clinics in HaematologyJournal of Clinical Pathology, 32
Nuclear Structure in Mutant L Cells
C. Stanners, J. Till (1960)
DNA synthesis in individual L-strain mouse cells.Biochimica et biophysica acta, 37
W. Nagl (1970)
Correlation of chromatin structure and interphase stage in nuclei of Allium flavum., 1
I. Dardick, G. Setterfield (1976)
Volume of condensed chromatin in developing primitive-line erythrocytes of chick.Experimental cell research, 100 1
(1971)
Cytological variations in the constitutive heterochromatin of Microtus agrestes. Chromosoma ( Berl
Y. Alvarez, Y. Valladares (1972)
Differential staining of the cell cycle.Nature: New biology, 238 87
K. Brasch, G. Setterfield (1974)
Structural organization of chromosomes in interphase nuclei.Experimental cell research, 83 1
Mutant lines of mouse L cells, TS A1S9, and TS C1, show temperature-sensitive (TS) DNA synthesis and cell division when shifted from 34 degrees to 38.5 degrees C. With TS A1S9 the decline in DNA synthesis begins after 6-8 h at 38.5 degrees C and is most marked at about 24 h. Most cells in S, G2, or M at temperature upshift complete one mitosis and accumulate in the subsequent interphase at G1 or early S as a result of expression of a primary defect, failure of elongation of newly made small DNA fragments. Heat inactivation of TS C1 cells is more rapid; they fail to complete the interphase in progress at temperature upshift and accumulate at late S or G2. Inhibition of both cell types is reversible on return to 34 degrees C. Cell and nuclear growth continues during inhibition of replication. Expression of both TS mutations leads to a marked change in gross organization of chromatin as revealed by electron microscopy. Nuclei of wild-type cells at 34 degrees and 38.5 degrees C and mutant cells at 34 degrees C show a range of aggregation of condensed chromatin from small dispersed bodies to large discrete clumps, with the majority in an intermediate state. In TS cells at 38.5 degrees C, condensed chromatin bodies in the central nuclear region become disaggregated into small clumps dispersed through the nucleus. Morphometric estimation of volume of condensed chromatin indicates that this process is not due to complete decondensation of chromatin fibrils, but rather involves dispersal of large condensed chromatin bodies into finer aggregates and loosening of fibrils within the aggregates. The dispersed condition is reversed in nuclei which resume DNA synthesis when TS cells are downshifted from 38.5 degrees to 34 degrees C. The morphological observations are consistent with the hypothesis that condensed chromatin normally undergoes an ordered cycle of transient, localized disaggregation and reaggregation associated with replication. In temperature-inactivated mutants, normal progressive disaggregation presumably occurs, but subsequent lack of chromatin replication prevents reaggregation.
The Journal of Cell Biology – Rockefeller University Press
Published: Apr 1, 1978
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