Access the full text.
Sign up today, get DeepDyve free for 14 days.
References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.
anlage was labeled by adding 400 Mc/ml thymidine- 3 H (Nuclear-Chicago Corp., Des Plaines, Ill. spec. act., 17, 2 mc/mM) to a culture of exconjugants during the first polyploidization stage (0-22 hr after separation of the conjugation pairs). After this, the exconjugants were washed three times with culture fluid, placed for 1 hr in fresh culture fluid, followed by 1 hr in culture fluid with FIGURE 1 Distribution of radioactivity of Stylonychia mytilus exconjugants. Each value represents 10 samples of 40 exconjugants each. The time of treatment with thymidine-311 is indicated; 0 = TCAinsoluble radioactive substances in A = TCA-solthe animals; A uble radioactive substances in the = radioactivity animals; lin the culture fluid. cpm, counts per minute. 10 20 Thymidine-3H-- 70 Hr macronuclear anlage develops to the definitive macronucleus (Ammermann, 1965). Scintillationcounter measurements showed that the DNA which leaves the anlage at the end of the giant chromosome stage does not stay in a TCA-insoluble form in the cytoplasm (Ammermann, 1968). However, it was not clear whether the DNA breakdown products remained in the cytoplasm in a TCA-soluble form or were released into the surrounding culture fluid. This question was resolved with a windowless gas flow counter.
The Journal of Cell Biology – Rockefeller University Press
Published: Feb 1, 1969
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.