Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

RELEASE OF DNA BREAKDOWN PRODUCTS INTO THE CULTURE MEDIUM OF STYLONYCHIA MYTILUS EXCONJUGANTS (PROTOZOA, CILIATA) DURING THE DESTRUCTION OF THE POLYTENE CHROMOSOMES

RELEASE OF DNA BREAKDOWN PRODUCTS INTO THE CULTURE MEDIUM OF STYLONYCHIA MYTILUS EXCONJUGANTS... anlage was labeled by adding 400 Mc/ml thymidine- 3 H (Nuclear-Chicago Corp., Des Plaines, Ill. spec. act., 17, 2 mc/mM) to a culture of exconjugants during the first polyploidization stage (0-22 hr after separation of the conjugation pairs). After this, the exconjugants were washed three times with culture fluid, placed for 1 hr in fresh culture fluid, followed by 1 hr in culture fluid with FIGURE 1 Distribution of radioactivity of Stylonychia mytilus exconjugants. Each value represents 10 samples of 40 exconjugants each. The time of treatment with thymidine-311 is indicated; 0 = TCAinsoluble radioactive substances in A = TCA-solthe animals; A uble radioactive substances in the = radioactivity animals; lin the culture fluid. cpm, counts per minute. 10 20 Thymidine-3H-- 70 Hr macronuclear anlage develops to the definitive macronucleus (Ammermann, 1965). Scintillationcounter measurements showed that the DNA which leaves the anlage at the end of the giant chromosome stage does not stay in a TCA-insoluble form in the cytoplasm (Ammermann, 1968). However, it was not clear whether the DNA breakdown products remained in the cytoplasm in a TCA-soluble form or were released into the surrounding culture fluid. This question was resolved with a windowless gas flow counter. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

RELEASE OF DNA BREAKDOWN PRODUCTS INTO THE CULTURE MEDIUM OF STYLONYCHIA MYTILUS EXCONJUGANTS (PROTOZOA, CILIATA) DURING THE DESTRUCTION OF THE POLYTENE CHROMOSOMES

The Journal of Cell Biology , Volume 40 (2): 576 – Feb 1, 1969

Loading next page...
 
/lp/rockefeller-university-press/release-of-dna-breakdown-products-into-the-culture-medium-of-IoGhcR2gWr

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Rockefeller University Press
Copyright
Copyright © 1969 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.40.2.576
Publisher site
See Article on Publisher Site

Abstract

anlage was labeled by adding 400 Mc/ml thymidine- 3 H (Nuclear-Chicago Corp., Des Plaines, Ill. spec. act., 17, 2 mc/mM) to a culture of exconjugants during the first polyploidization stage (0-22 hr after separation of the conjugation pairs). After this, the exconjugants were washed three times with culture fluid, placed for 1 hr in fresh culture fluid, followed by 1 hr in culture fluid with FIGURE 1 Distribution of radioactivity of Stylonychia mytilus exconjugants. Each value represents 10 samples of 40 exconjugants each. The time of treatment with thymidine-311 is indicated; 0 = TCAinsoluble radioactive substances in A = TCA-solthe animals; A uble radioactive substances in the = radioactivity animals; lin the culture fluid. cpm, counts per minute. 10 20 Thymidine-3H-- 70 Hr macronuclear anlage develops to the definitive macronucleus (Ammermann, 1965). Scintillationcounter measurements showed that the DNA which leaves the anlage at the end of the giant chromosome stage does not stay in a TCA-insoluble form in the cytoplasm (Ammermann, 1968). However, it was not clear whether the DNA breakdown products remained in the cytoplasm in a TCA-soluble form or were released into the surrounding culture fluid. This question was resolved with a windowless gas flow counter.

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Feb 1, 1969

There are no references for this article.