Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Berlin, J. Fera (1977)
Changes in membrane microviscosity associated with phagocytosis: effects of colchicine.Proceedings of the National Academy of Sciences of the United States of America, 74 3
J. Oliver, T. Ukena, R. Berlin (1974)
Effects of phagocytosis and colchicine on the distribution of lectin-binding sites on cell surfaces.Proceedings of the National Academy of Sciences of the United States of America, 71 2
S. Malawista, P. Bodel (1967)
The dissociation by colchicine of phagocytosis from increased oxygen consumption in human leukocytes.The Journal of clinical investigation, 46 5
J. Oliver (1976)
Concanavalin A cap formation on human polymorphonuclear leukocytes induced by R17934, a new antitumor drug that interferes with microtubule assembly.Journal of the Reticuloendothelial Society, 19 6
S. Malawista (1975)
The action of colchicine in acute gouty arthritis.Arthritis and rheumatism, 18 6 Suppl
S. Malawista, K. Bensch (1967)
Human Polymorphonuclear Leukocytes: Demonstration of Microtubules and Effect of ColchicineScience, 156
R. Zurier, S. Hoffstein, G. Weissmann (1973)
MECHANISMS OF LYSOSOMAL ENZYME RELEASE FROM HUMAN LEUKOCYTESThe Journal of Cell Biology, 58
Barry Brown, Albano Jd, Ekins Rp, A. Sgherzi (1971)
A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate.The Biochemical journal, 121 3
J. Oliver (1976)
Impaired microtubule function correctable by cyclic GMP and cholinergic agonists in the Chediak-Higashi syndrome.The American journal of pathology, 85 2
S. Rudolph, P. Greengard, S. Malawista (1977)
Effects of colchicine on cyclic AMP levels in human leukocytes.Proceedings of the National Academy of Sciences of the United States of America, 74 8
J. Oliver, D. Albertini, R. Berlin (1976)
Effects of glutathione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophilsThe Journal of Cell Biology, 71
T. Ukena, R. Berlin (1972)
EFFECT OF COLCHICINE AND VINBLASTINE ON THE TOPOGRAPHICAL SEPARATION OF MEMBRANE FUNCTIONSThe Journal of Experimental Medicine, 136
L. Ignarro, W. George (1974)
Hormonal control of lysosomal enzyme release from human neutrophils: elevation of cyclic nucleotide levels by autonomic neurohormones.Proceedings of the National Academy of Sciences of the United States of America, 71 5
S. Malawista (1975)
MICROTUBULES AND THE MOBILIZATION OF LYSOSOMES IN PHAGOCYTIZING HUMAN LEUKOCYTES *Annals of the New York Academy of Sciences, 253
(1978)
886 TrIE JOURNAL OF CELL BIOLOGY
G. Schreiner, E. Unanue (1976)
Membrane and cytoplasmic changes in B lymphocytes induced by ligand-surface immunoglobulin interaction.Advances in immunology, 24
D. Albertini, R. Berlin, J. Oliver (1977)
The mechanism of concanavalin A cap formation in leukocytes.Journal of cell science, 26
L. Boxer, A. Watanabe, Manfred Rister, H. Besch, Allen Jm, R. Baehner (1976)
Correction of leukocyte function in Chediak-Higashi syndrome by ascorbate.The New England journal of medicine, 295 19
G. Weissmann, I. Goldstein, S. Hoffstein, P. Tsung (1975)
RECIPROCAL EFFECTS OF cAMP AND cGMP ON MICROTUBULE‐DEPENDENT RELEASE OF LYSOSOMAL ENZYMES *Annals of the New York Academy of Sciences, 253
J. Clin. Invest
We have shown previously that the β-adrenergic agonist isoproterenol (2μM) and the phosphodiesterase inhibitor isobutylmethylxanthine (1 mM) produce a much greater increase in cyclic AMP in human leukocytes that have been pretreated with colchicine (or with other agents that affect microtubule assembly) than in control leukocytes. The effects of colchicines were both time- and dose-dependant. These and other data suggested that the generation of cyclic AMP is normally restricted by an intact system of cytoplasmic microtubules. If so, then the same time and dose dependencies might apply to other colchicines-induced changes in leukocyte function. We have now assayed the distribution of concanavalin A (Con A)-receptor complexes on the leukocyte membrane, taking into account that leukocytes competent to assemble microtubules show a uniform distribution of surface- bound Con A whereas microtubule-deficient cells accumulate Con A in surface caps. We have found that the effect of colchicine on capping is also both time- and dose dependent, and that the dose-response relationships conform to those required to increase cyclic AMP levels. These findings provide further evidence that both colchicine-induced Con-A capping and colchicine- induced cyclic AMP generation depend upon the relaxation of constraints normally imposed by cytoplasmic microtubules upon the plasma membrane, which limit, respectively, lateral mobility of the lectin-receptor complexes, and expression of hormone-sensitive adenylate cyclase. Moreover, colchicine-induced Con-A cap formation is not affected even by very large changes in leukocyte cyclic AMP levels. Thus, elevated cyclic AMP levels do not appear to promote the dissolution of microtubules; rather, the dissolution of microtubules permits the generation of increased amounts of cyclic AMP.
The Journal of Cell Biology – Rockefeller University Press
Published: Jun 1, 1978
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.