Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Fluorescent microtubules break up under illumination.

Fluorescent microtubules break up under illumination. We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show that all four analogues assemble into microtubules which break up when exposed to light of the wavelengths that excite fluorescence. This sensitivity places severe constraints on the use of these analogues in studies of microtubule dynamics. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

Fluorescent microtubules break up under illumination.

The Journal of Cell Biology , Volume 107 (3): 1011 – Sep 1, 1988

Loading next page...
 
/lp/rockefeller-university-press/fluorescent-microtubules-break-up-under-illumination-vdyxGq50dh

References (28)

Publisher
Rockefeller University Press
Copyright
© 1988 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.107.3.1011
Publisher site
See Article on Publisher Site

Abstract

We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show that all four analogues assemble into microtubules which break up when exposed to light of the wavelengths that excite fluorescence. This sensitivity places severe constraints on the use of these analogues in studies of microtubule dynamics.

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Sep 1, 1988

There are no references for this article.