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EFFECTS OF CORTISOL ON CULTURED RAT HEART CELLS

EFFECTS OF CORTISOL ON CULTURED RAT HEART CELLS This paper reports the determination of the ability of rat heart cells in culture to release 14 Cpalmitate from its triglyceride and to oxidize this fatty acid and free 14 Cpalmitate to 14 CO 2 when the cells are actively beating and when they stop beating after aging in culture. In addition, the levels of glucose, glycogen, and ATP were determined to relate the concentration of these metabolites with beating and with cessation of beating. When young rat heart cells in culture are actively beating, they oxidize free fatty acids at a rate parallel with cellular ATP production. Both fatty acid oxidation and ATP production remain constant while the cells continue to beat. Furthermore, glucose is removed from the growth medium by the cells and stored as glycogen. When cultured cells stop beating, a decrease is seen in their ability to oxidize free fatty acids and to release them from their corresponding triglycerides. Concomitant with decreased fatty acid oxidation is a decrease in cellular levels of ATP until beating ceases. Midway between initiation of cultures and cessation of beating the cells begin to mobilize the stored glycogen. When the growth medium is supplemented with cortisol acetate and given to cultures which have ceased to beat, reinitiation of beating occurs. Furthermore, all decreases previously observed in ATP levels, fatty acid oxidation, and esterase activity are restored. Footnotes Submitted: 26 July 1972 Revision received 11 December 1972 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

EFFECTS OF CORTISOL ON CULTURED RAT HEART CELLS

The Journal of Cell Biology , Volume 57 (1): 109 – Apr 1, 1973

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Publisher
Rockefeller University Press
Copyright
© 1973 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.57.1.109
Publisher site
See Article on Publisher Site

Abstract

This paper reports the determination of the ability of rat heart cells in culture to release 14 Cpalmitate from its triglyceride and to oxidize this fatty acid and free 14 Cpalmitate to 14 CO 2 when the cells are actively beating and when they stop beating after aging in culture. In addition, the levels of glucose, glycogen, and ATP were determined to relate the concentration of these metabolites with beating and with cessation of beating. When young rat heart cells in culture are actively beating, they oxidize free fatty acids at a rate parallel with cellular ATP production. Both fatty acid oxidation and ATP production remain constant while the cells continue to beat. Furthermore, glucose is removed from the growth medium by the cells and stored as glycogen. When cultured cells stop beating, a decrease is seen in their ability to oxidize free fatty acids and to release them from their corresponding triglycerides. Concomitant with decreased fatty acid oxidation is a decrease in cellular levels of ATP until beating ceases. Midway between initiation of cultures and cessation of beating the cells begin to mobilize the stored glycogen. When the growth medium is supplemented with cortisol acetate and given to cultures which have ceased to beat, reinitiation of beating occurs. Furthermore, all decreases previously observed in ATP levels, fatty acid oxidation, and esterase activity are restored. Footnotes Submitted: 26 July 1972 Revision received 11 December 1972

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Apr 1, 1973

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