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THE JOURNAL OF CELL BIOLOGY . Volume 47, 1970 ⢠pages 782 -786 FIGURE 1 An electron micrograph showing a portion of a gastric gland which was fixed and treated with lanthanum immediately after excision of the tissue . Lanthanum completely outlines the oxyntic cells as well as an adjacent mucous neck cell (MNC), except for tight junctional regions (TJ) . The heavy metal tracer can be seen in the gland lumen (L), between the apical cytoplasmic extensions, along the basal border, and in the basal infoldings (BF) . Stained with uranyl and lead salts . X 14,000 . does not penetrate the membranous systems within the cells . Thus it would appear that under these conditions of fixation the tubular system of oxyntic cells is not directly continuous with the free surface . In very rare cases (three cells out of many examined) an intense darkening of the ground substance of the cytoplasm occurred, but the tubular lumina remained free of the tracer . These cells were undoubtedly damaged, and became freely accessible to lanthanum in a manner similar to that described by Revel and Karnovsky (6), and Ritch and Philpott (9) . We have frequently found
The Journal of Cell Biology – Rockefeller University Press
Published: Dec 1, 1970
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