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ABSTRACT A n u m b e r o f instantaneous changes occurred when picrate was a d d e d to a suspension o f human red cells in stead)' state with respect to glycolysis and ion distribution across the m e m b r a n e at p H 7.40. T h e rate o f glycolysis increased, without change in glycolytic quotient, to a new steady-state value, the effect reaching a m a x i m u m o f 1.75 times the rate o f the control at 0.5 mM picrate. Inorganic phosphate (Pi) was released at a relatively constant rate, increasing with picrate concentration to 1.0 mmol Pl/liter cells x h at 5-6 mM picrate. T h e steadystate concentration o f A T P and 1,3-diphosphoglycerate (1,3-DPG) decreased to new stable values within 15-45 rain after the addition o f picrate. T h e A T P level was affected only at picrate concentrations o f I mM or more, and the level o f A T P stabilized at 75% of the control values at 4 mM o f picrate. In contrast, 1,3-DPG concentrations decreased to 40% o f the
The Journal of General Physiology – Rockefeller University Press
Published: Nov 1, 1977
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