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A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL

A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10 –7 cm. 2 /sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution. Footnotes Submitted: 22 October 1940 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of General Physiology Rockefeller University Press

A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL

The Journal of General Physiology , Volume 24 (4): 459 – Mar 20, 1941

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References (4)

Publisher
Rockefeller University Press
Copyright
© 1941 Rockefeller University Press
ISSN
0022-1295
eISSN
1540-7748
DOI
10.1085/jgp.24.4.459
Publisher site
See Article on Publisher Site

Abstract

A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10 –7 cm. 2 /sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution. Footnotes Submitted: 22 October 1940

Journal

The Journal of General PhysiologyRockefeller University Press

Published: Mar 20, 1941

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