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Gen. Physiol
(1939)
J. Biol. Chem
(1937)
Biol. Chem
J. Philpot (1943)
The UltracentrifugeNature, 151
A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined. The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10 –7 cm. 2 /sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution. Footnotes Submitted: 22 October 1940
The Journal of General Physiology – Rockefeller University Press
Published: Mar 20, 1941
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