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A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL

A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL 1. A method for measuring the O 2 consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas, representing the O 2 consumed, is followed. 2. The possible errors of the technique are appraised. 3. A single Actinosphaerium eichhornii consumes 0.00113 mm. 3 of O 2 per hour. A single Paramecium caudatum consumes 0.00049 mm. 3 of O 2 per hour. 4. The significance of the results and the limitations of the method are discussed. Footnotes Accepted: 19 November 1930 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of General Physiology Rockefeller University Press

A METHOD FOR DETERMINING THE OXYGEN CONSUMPTION OF A SINGLE CELL

The Journal of General Physiology , Volume 14 (3): 339 – Jan 20, 1931

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Publisher
Rockefeller University Press
Copyright
© 1931 Rockefeller University Press
ISSN
0022-1295
eISSN
1540-7748
DOI
10.1085/jgp.14.3.339
Publisher site
See Article on Publisher Site

Abstract

1. A method for measuring the O 2 consumption of a single cell is described. The cell is placed in a capillary tube adjacent to a bubble of air. KOH (5 per cent) is drawn in on the opposite side of the air and both ends of the tube are sealed with mineral oil. The decrease in the volume of the gas, representing the O 2 consumed, is followed. 2. The possible errors of the technique are appraised. 3. A single Actinosphaerium eichhornii consumes 0.00113 mm. 3 of O 2 per hour. A single Paramecium caudatum consumes 0.00049 mm. 3 of O 2 per hour. 4. The significance of the results and the limitations of the method are discussed. Footnotes Accepted: 19 November 1930

Journal

The Journal of General PhysiologyRockefeller University Press

Published: Jan 20, 1931

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