Subscribe to thousands of academic journals for just $40/month
Read and share the articles you need for your research, all in one place.

The two eIF4A helicases in Trypanosoma brucei are functionally distinct

Nucleic Acids Research , Volume 34 (9): 2495 – Jan 1, 2006

Details

Publisher
Oxford University Press
Copyright
Copyright © 2010 Oxford University Press
ISSN
0305-1048
eISSN
1362-4962
D.O.I.
10.1093/nar/gkl290
Publisher site
See Article on Publisher Site

Preview Only

Expand Tray Hide Tray

The two eIF4A helicases in Trypanosoma brucei are functionally distinct

Abstract

Abstract Protozoan parasites belonging to the family Trypanosomatidae are characterized by an unusual pathway for the production of mRNAs via polycistronic transcription and trans -splicing of a 5′ capped mini-exon which is linked to the 3′ cleavage and polyadenylation of the upstream transcript. However, little is known of the mechanism of protein synthesis in these organisms, despite their importance as agents of a number of human diseases. Here we have investigated the role of two Trypanosoma brucei homologues of the translation initiation factor eIF4A (in the light of subsequent experiments these were named as Tb EIF4AI and Tb EIF4AIII). eIF4A, a DEAD-box RNA helicase, is a subunit of the translation initiation complex eIF4F which binds to the cap structure of eukaryotic mRNA and recruits the small ribosomal subunit. Tb EIF4AI is a very abundant predominantly cytoplasmic protein (over 1 × 10 5 molecules/cell) and depletion to ∼10% of normal levels through RNA interference dramatically reduces protein synthesis one cell cycle following double-stranded RNA induction and stops cell proliferation. In contrast, Tb EIF4AIII is a nuclear, moderately expressed protein (∼1–2 × 10 4 molecules/cell), and its depletion stops cellular proliferation after approximately four cell cycles. Ectopic expression of a dominant negative mutant of Tb EIF4AI, but not of Tb EIF4AIII, induced a slow growth phenotype in transfected cells. Overall, our results suggest that only Tb EIF4AI is involved in protein synthesis while the properties and sequence of Tb EIF4AIII indicate that it may be the orthologue of eIF4AIII, a component of the exon junction complex in mammalian cells. © The Author 2006. Published by Oxford University Press. All rights reserved The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Loading next page...

Preview Only. This article cannot be rented because we do not currently have permission from the publisher.

 
/lp/oxford-university-press/the-two-eif4a-helicases-in-trypanosoma-brucei-are-functionally-GXXy16LgYC