Access the full text.
Sign up today, get DeepDyve free for 14 days.
Ewan Smith, S. Finn, Andrew Tee, G. Browne, C. Proud (2005)
The Tuberous Sclerosis Protein TSC2 Is Not Required for the Regulation of the Mammalian Target of Rapamycin by Amino Acids and Certain Cellular Stresses*Journal of Biological Chemistry, 280
Wenli Yang, A. Tabancay, J. Urano, F. Tamanoi (2001)
Failure to farnesylate Rheb protein contributes to the enrichment of G0/G1 phase cells in the Schizosaccharomyces pombe farnesyltransferase mutantMolecular Microbiology, 41
T. Plank, Raymond Yeung, E. Henske (1998)
Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene, interacts with tuberin and appears to be localized to cytoplasmic vesicles.Cancer research, 58 21
Andrew Tee, B. Manning, Philippe Roux, L. Cantley, J. Blenis (2003)
Tuberous Sclerosis Complex Gene Products, Tuberin and Hamartin, Control mTOR Signaling by Acting as a GTPase-Activating Protein Complex toward RhebCurrent Biology, 13
M. Slegtenhorst, E. Carr, R. Stoyanova, W. Kruger, E. Henske (2004)
Tsc1+ and tsc2+ Regulate Arginine Uptake and Metabolism in Schizosaccharomyces pombe*Journal of Biological Chemistry, 279
L. Saucedo, Xinsheng Gao, Dominic Chiarelli, Ling Li, Duoija Pan, B. Edgar (2003)
Rheb promotes cell growth as a component of the insulin/TOR signalling networkNature Cell Biology, 5
K. Tanaka, H. Okayama (2000)
A pcl-like cyclin activates the Res2p-Cdc10p cell cycle "start" transcriptional factor complex in fission yeast.Molecular biology of the cell, 11 9
M. Slegtenhorst, M. Nellist, B. Nagelkerken, J. Cheadle, R. Snell, Ans Ouweland, A. Reuser, J. Sampson, D. Halley, P. Sluijs (1998)
Interaction between hamartin and tuberin, the TSC1 and TSC2 gene products.Human molecular genetics, 7 6
K. Inoki, Tianqing Zhu, K. Guan (2003)
TSC2 Mediates Cellular Energy Response to Control Cell Growth and SurvivalCell, 115
Samuel Long, S. Ortiz-Vega, Yenshou Lin, J. Avruch (2005)
Rheb Binding to Mammalian Target of Rapamycin (mTOR) Is Regulated by Amino Acid Sufficiency*Journal of Biological Chemistry, 280
Samuel Long, Yenshou Lin, S. Ortiz-Vega, K. Yonezawa, J. Avruch (2005)
Rheb Binds and Regulates the mTOR KinaseCurrent Biology, 15
Hugo Stocker, T. Radimerski, B. Schindelholz, F. Wittwer, Priyanka Belawat, P. Daram, S. Breuer, G. Thomas, E. Hafen (2003)
Rheb is an essential regulator of S6K in controlling cell growth in DrosophilaNature Cell Biology, 5
Yong Zhang, Xinsheng Gao, L. Saucedo, B. Ru, B. Edgar, D. Pan (2003)
Rheb is a direct target of the tuberous sclerosis tumour suppressor proteinsNature Cell Biology, 5
Kathleen Mach, Kyle Furge, Charles Albright (2000)
Loss of Rhb1, a Rheb-related GTPase in fission yeast, causes growth arrest with a terminal phenotype similar to that caused by nitrogen starvation.Genetics, 155 2
J. Urano, A. Tabancay, Wenli Yang, F. Tamanoi (2000)
The Saccharomyces cerevisiae Rheb G-protein Is Involved in Regulating Canavanine Resistance and Arginine Uptake*The Journal of Biological Chemistry, 275
K. Inoki, M. Corradetti, K. Guan (2004)
Dysregulation of the TSC-mTOR pathway in human diseaseNature Genetics, 37
Wenli Yang, J. Urano, F. Tamanoi (2000)
Protein Farnesylation Is Critical for Maintaining Normal Cell Morphology and Canavanine Resistance in Schizosaccharomyces pombe *The Journal of Biological Chemistry, 275
N. Tapon, N. Ito, B. Dickson, J. Treisman, I. Hariharan (2001)
The Drosophila Tuberous Sclerosis Complex Gene Homologs Restrict Cell Growth and Cell ProliferationCell, 105
A. Castro, J. Rebhun, G. Clark, L. Quilliam (2003)
Rheb Binds Tuberous Sclerosis Complex 2 (TSC2) and Promotes S6 Kinase Activation in a Rapamycin- and Farnesylation-dependent Manner*Journal of Biological Chemistry, 278
E. Jacinto, M. Hall (2003)
TOR signalling in bugs, brain and brawnNature Reviews Molecular Cell Biology, 4
(1993)
Identification and characterization of the tuberous sclerosis gene on chromosome 16 . The European Chromosome 16 Tuberous Sclerosis Consortium
S. Matsumoto, A. Bandyopadhyay, D. Kwiatkowski, U. Maitra, Tomohiro Matsumoto (2002)
Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe.Genetics, 161 3
B. Manning, L. Cantley (2003)
Rheb fills a GAP between TSC and TOR.Trends in biochemical sciences, 28 11
S. Uyeda, J. Sclater, M. Slegtenhorst, R. Hoogt, C. Hermans, M. Nellist, B. Janssen, S. Verhoef, D. Lindhout, A. Ouweland, D. Halley, Janet Young, Mariwyn Burley, Steve Jeremiah, K. Woodward, J. Nahmias, M. Fox, R. Ekong, J. Osborne, J. Wolfe, S. Povey, R. Snell, J. Cheadle, A. Jones, Maria Tachataki, D. Ravine, J. Sampson, M. Reeve, Paul Richardson, Friederike Wilmer, Cheryl Munro, T. Hawkins, T. Sepp, S. Ward, A. Green, J. Yates, J. Kwiatkowska, E. Henske, M. Short, J. Haines, S. Jóźwiak, D. Kwiatkowski (1997)
Identification of the tuberous sclerosis gene TSC1 on chromosome 9q34.Science, 277 5327
A. Tabancay, C. Gau, Iara Machado, Erik Uhlmann, D. Gutmann, Lea Guo, F. Tamanoi (2003)
Identification of Dominant Negative Mutants of Rheb GTPase and Their Use to Implicate the Involvement of Human Rheb in the Activation of p70S6K*Journal of Biological Chemistry, 278
U. Leupold (1970)
Chapter 8 Genetical Methods for Schizosaccharomyces pombeMethods in Cell Biology, 4
(2002)
Role of the Tsc 1 – Tsc 2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe
C. Potter, He Huang, Tian Xu (2001)
Drosophila Tsc1 Functions with Tsc2 to Antagonize Insulin Signaling in Regulating Cell Growth, Cell Proliferation, and Organ SizeCell, 105
T. Soucek, M. Rosner, A. Miloloza, M. Kubista, J. Cheadle, J. Sampson, M. Hengstschläger (2001)
Tuberous sclerosis causing mutants of the TSC2 gene product affect proliferation and p27 expressionOncogene, 20
A. Garami, F. Zwartkruis, T. Nobukuni, M. Joaquin, M. Roccio, Hugo Stocker, S. Kozma, E. Hafen, J. Bos, G. Thomas (2003)
Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2.Molecular cell, 11 6
M. Nellist, B. Janssen, P. Brook-Carter, A. Hesseling-Janssen, M. Maheshwar, S. Verhoef, Ans Ouweland, D. Lindhout, B. Eussen, I. Cordeiro, H. Santos, D. Halley, J. Sampson, C. Ward, B. Peral, S. Thomas, J. Hughes, P. Harris, J. Roelfsema, J. Saris, L. Spruit, D. Peters, J. Dauwerse, M. Bruening (1993)
Identification and characterization of the tuberous sclerosis gene on chromosome 16Cell, 75
Tuberous sclerosis complex is a tumor suppressor syndrome caused by mutations in either the TSC1 or the TSC2 gene. Previous studies have shown that deletion of the TSC1 or TSC2 ortholog in Schizosaccharomyces pombe results in an amino acid uptake defect, with conditional lethality. We identified a G1 cyclin, pas1+, as a high-copy suppressor of this defect in Δtsc1. Disruption of pas1+ causes defects in arginine and leucine uptake that are remarkably similar to Δtsc1 and Δtsc2, whereas Δpas1Δtsc1 and Δpas1Δtsc2 double mutants have more severe amino acid uptake defects. In a second screen, we identified a novel G63D/S165 N mutant of the small GTPase Rhb1, the target of the Tsc1/Tsc2 protein complex. The Rhb1 mutant suppresses amino acid uptake in Δtsc1 yeast, but not in Δpas1 yeast. Hence, Pas1 does not regulate amino acid uptake through Rhb1. To determine whether Pas1 links nutrient availability to cell cycle progression downstream of the Tsc1/Tsc2 complex, we examined the kinetics of G1 arrest in single and double mutant strains. After nitrogen starvation, Δtsc1 and Δtsc2 yeast had a delay in G1 arrest when compared with wild-type, which was rescued by deletion of pas1+. In summary, we identified the G1 cyclin, Pas1, as a novel regulator of amino acid uptake. Our data support a model in which Pas1 inhibits G1 arrest downstream of Tsc1 and Tsc2, linking nutrient uptake and cell cycle progression in yeast.
Human Molecular Genetics – Oxford University Press
Published: Oct 1, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.