Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Bennett, J. Smith (1976)
Nuclear dna amounts in angiosperms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 274 933
Y. Jacob, C. Teyssier, S. Reynders-Aloisi, S. Brown (1996)
USE OF FLOW CYTOMETRY FOR THE RAPID DETERMINATION OF PLOIDY LEVEL IN THE GENUS ROSA
E. Dickson, K. Arumuganathan, S. Kresovich, J. Doyle (1992)
NUCLEAR DNA CONTENT VARIATION WITHIN THE ROSACEAEAmerican Journal of Botany, 79
Michael Bennett, I. Leitch (1995)
Nuclear DNA Amounts in AngiospermsAnnals of Botany, 76
C. Hurst (1941)
Notes on the origin and evolution of our garden roses 11, 66
I. O'brien, Dale Smith, R. Gardner, B. Murray (1996)
Flow cytometric determination of genome size in PinusPlant Science, 115
M. El-Lakany (1972)
QUANTITATIVE VARIATION IN DNA AS RELATED TO PLOIDY LEVEL AND SPECIES IN SOME WILD ROSESCanadian journal of genetics and cytology, 14
J. Doležel, S. Sgorbati, S. Lucretti (1992)
Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plantsPhysiologia Plantarum, 85
C. Darlington, A. Wylie (1956)
Chromosome atlas of flowering plants.Kew Bulletin, 11
A. Moyne, F. Souq, L. Yean, S. Brown, M. Boulay, B. Sangwan-Norreel (1993)
Relationship between cell ploidy and regeneration capacity of long term Rosa hybrida culturesPlant Science, 93
H. Tauber (1956)
Methods of Enzymology.Journal of the American Chemical Society, 78
C. Bagwell, D. Baker, S. Whetstone, M. Munson, S. Hitchcox, K. Ault, E. Lovett (1989)
A simple and rapid method for determining the linearity of a flow cytometer amplification system.Cytometry, 10 6
D. Walker (1971)
[18] Chloroplasts (and Grana): Aqueous (including high carbon fixation ability)Methods in Enzymology, 23
Nuclei isolated from young leaves were stained with propidium iodide (PI) and their fluorescence intensities were measured by flow cytometry. The ratio of fluorescence intensities of four calibration standards and 34 roses to an internal standard, parsley (Petroselinum crispum), provided a basis for estimating the DNA amounts of P. crispum and rose. The 2C DNA amount of P. crispum(2 n = 22) was estimated as 4.46 pg (s.d. ± 0.08 pg). The 2C DNA amounts of diploid roses (2n = 14) varied between subgenera, sections and cultivars, and ranged from 0.78 pg (s.d. ± 0.08 pg) in Rosa xanthina and R. sericea(section Pimpinellifoliae) to 1.29 pg (s.d. ± 0.08 pg) in ‘Félicité et Perpétue’ (Hybrid Sempervirens). Within each section, the DNA amounts of diploid species were similar. In the sections Carolinae and Cinnamomeae, DNA amounts were proportional to ploidy numbers. In the Pimpinellifoliae, DNA amounts of tetraploids were disproportionately larger than those of diploids which suggests that they originated as hybrids with species of sections with larger DNA amounts. Ratios of the fluorescence intensities of nuclei of roses to P. crispum(internal standard) were also measured using 4′,6-diamidino-2-phenylindole (DAPI) which binds preferentially to AT base pairs. These DAPI ratios were lower than, but closely correlated (r2 = 0.997) with PI ratios. Fluorescence intensities of either PI or DAPI-stained nuclei of roses can be used as rapid indicators of ploidy if variation in the DNA amounts between different taxonomic groups is taken into account. Copyright 2000 Annals of Botany Company
Annals of Botany – Oxford University Press
Published: Apr 1, 2000
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.