Molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a Myb protein from the protozoan parasite Trichomonas vaginalis
Abstract
Abstract Iron-inducible transcription of the ap65-1 gene in Trichomonas vaginalis involves at least three Myb-like transcriptional factors ( tv Myb1, tv Myb2 and tv Myb3) that differentially bind to two closely spaced promoter sites, MRE-1/MRE-2r and MRE-2f. Here, we defined a fragment of tv Myb2 comprising residues 40–156 ( tv Myb2 40–156 ) as the minimum structural unit that retains near full binding affinity with the promoter DNAs. Like c-Myb in vertebrates, the DNA-free tv Myb2 40–156 has a flexible and open conformation. Upon binding to the promoter DNA elements, tv Myb2 40–156 undergoes significant conformational re-arrangement and structure stabilization. Crystal structures of tv Myb2 40–156 in complex with promoter element-containing DNA oligomers showed that 5′-a/gACGAT-3′ is the specific base sequence recognized by tv Myb2 40–156 , which does not fully conform to that of the Myb binding site sequence. Furthermore, Lys 49 , which is upstream of the R2 motif (amino acids 52–102) also participates in specific DNA sequence recognition. Intriguingly, tv Myb2 40–156 binds to the promoter elements in an orientation opposite to that proposed in the HADDOCK model of the tv Myb1 35–141 /MRE-1-MRE-2r complex. These results shed new light on understanding the molecular mechanism of Myb–DNA recognition and provide a framework to study the molecular basis of transcriptional regulation of myriad Mybs in T. vaginalis . © The Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. « Previous | Next Article » Table of Contents This Article Nucl. Acids Res. (2011) 39 (20): 8992-9008. doi: 10.1093/nar/gkr558 First published online: July 19, 2011 This article is Open Access OA » Abstract Free Full Text (HTML) Free Full Text (PDF) Free Screen PDF Supplementary Data All Versions of this Article: gkr558v1 39/20/8992 most recent Classifications Structural Biology Services Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in Web of Science Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Citing articles via Web of Science Google Scholar Articles by Jiang, I. Articles by Huang, T. PubMed PubMed citation Articles by Jiang, I. Articles by Huang, T. Related Content Load related web page information Share Email this article CiteULike Connotea Delicious Facebook Google+ Mendeley Twitter What's this? 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