Gene List on a Plant Tumor-inducing Plasmid, pTi-SAKURA in Agrobacterium tumefaciens MAFF301001
Abstract
Yoshiyuki HATTORI, 1 '* Misugi URAJI, 1 ^ Katsunori SUZUKI,1'* Nobuyuki OHTA, 1 Kumi IWATA,1 Akira KATO, 2 and Kazuo YOSHIDA1'* Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan1 and Hokkaido Agricultural Experiment Station, Sapporo 061-8555, Japan2 (Received 2 October 2000; revised 11 October 2000) Downloaded from dnaresearch.oxfordjournals.org at Infovell on February 16, 2011 Plant infectious Agrobacteria harbor large plasmids Ti or Ri (about 200 kb), which induce crown gall or hairy root syndromes,1 respectively. These plasmids have three important functional regions, transfer DNA (T-DNA) region, virulence (Vir) region and conjugation transfer (Tra) region. The T-DNA region is integrated into plant chromosomes, and the subsequent expression of its plant growth hormone genes causes the formation of a crown gall or hairy root tumor.2 The Vir region is specifically responsible for processing T-DNA and transfer into plant chromosomes.3 The Tra region is involved in bacterial conjugation. These gene systems have provided the fundamental bases of plant genetic engineering. Recently, we have succeeded in the first complete sequencing of a Ti plasmid and its short report has been published.4 In this paper, we describe the list of genes on the plasmid, pTi-SAKURA in A. tumefadens