Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment

Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure... BACKGROUNDWe have previously suggested that the Toluidine Blue (TB) test can be used for sperm chromatin structure assessment. In this study, we wished to evaluate the clinical applicability of the TB test in assessing male fertility potential using well-defined groups of fertile and infertile men.METHODSSixty-three fertile and 79 infertile men were tested. Infertility thresholds for the proportion of sperm with abnormal [TB dark cells (TBDCs)] and normal [TB light cells (TBLCs)] chromatin structure were set by the ROC curve analysis.RESULTSThresholds of 45 TBDC and 20 TBLC were highly predictive for infertility (specificity of the test: 92 and 90, respectively), but they were poor predictors of the fertility (sensitivity of the test: 42 and 32, respectively). Odds ratio for infertility was 7.5 [95 confidence interval (CI): 2.720.8] when the 45 TBDC threshold was used and 4.4 (95 CI: 1.711.6) when the 20 TBLC threshold was used.CONCLUSIONSThe TB test can be suggested for clinical use as a complementary test for standard semen analysis to diagnose male infertility. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Reproduction Oxford University Press

Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment

Loading next page...
 
/lp/oxford-university-press/evaluation-of-male-fertility-potential-by-toluidine-blue-test-for-0N0ic5PypN

References (34)

Publisher
Oxford University Press
Copyright
© Published by Oxford University Press.
Subject
ORIGINAL ARTICLES
ISSN
0268-1161
eISSN
1460-2350
DOI
10.1093/humrep/dep068
pmid
19304993
Publisher site
See Article on Publisher Site

Abstract

BACKGROUNDWe have previously suggested that the Toluidine Blue (TB) test can be used for sperm chromatin structure assessment. In this study, we wished to evaluate the clinical applicability of the TB test in assessing male fertility potential using well-defined groups of fertile and infertile men.METHODSSixty-three fertile and 79 infertile men were tested. Infertility thresholds for the proportion of sperm with abnormal [TB dark cells (TBDCs)] and normal [TB light cells (TBLCs)] chromatin structure were set by the ROC curve analysis.RESULTSThresholds of 45 TBDC and 20 TBLC were highly predictive for infertility (specificity of the test: 92 and 90, respectively), but they were poor predictors of the fertility (sensitivity of the test: 42 and 32, respectively). Odds ratio for infertility was 7.5 [95 confidence interval (CI): 2.720.8] when the 45 TBDC threshold was used and 4.4 (95 CI: 1.711.6) when the 20 TBLC threshold was used.CONCLUSIONSThe TB test can be suggested for clinical use as a complementary test for standard semen analysis to diagnose male infertility.

Journal

Human ReproductionOxford University Press

Published: Jul 21, 2009

Keywords: Toluidine Blue sperm chromatin male infertility diagnostic test

There are no references for this article.