Subscribe to thousands of academic journals for just $40/month
Read and share the articles you need for your research, all in one place.

Elevation of 8-Hydroxydeoxyguanosine in DNA from Isolated Mouse Lung Cells Following In Vivo Treatment with Aflatoxin B1

Toxicological Sciences , Volume 98 (1): 57 – Jul 1, 2007

Details

Publisher
Oxford University Press
Copyright
Copyright © 2010 Society of Toxicology
ISSN
1096-6080
eISSN
1096-0929
D.O.I.
10.1093/toxsci/kfm073
Publisher site
See Article on Publisher Site

Preview Only

Expand Tray Hide Tray

Elevation of 8-Hydroxydeoxyguanosine in DNA from Isolated Mouse Lung Cells Following In Vivo Treatment with Aflatoxin B1

Abstract

Abstract Aflatoxin B 1 (AFB 1 ) is a mycotoxin produced by some strains of Aspergillus and is a recognized pulmonary and hepatic carcinogen. The most widely accepted mechanism of AFB 1 carcinogenicity involves bioactivation to AFB 1 -8,9- exo -epoxide and binding to DNA to form AFB 1 -N 7 -guanine. Another potential cause of DNA damage is AFB 1 -mediated stimulation of reactive oxygen species formation, leading to oxidation of DNA bases. The objective of this study was to determine the ability of AFB 1 to cause oxidative DNA damage in lung cell types of the A/J mouse. The formation of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in freshly isolated mouse lung alveolar macrophages, alveolar type II cells, and nonciliated bronchial epithelial (Clara) cells was assessed by high-performance liquid chromatography with electrochemical detection. An approximately 3-fold increase in 8-OHdG formation occurred in both alveolar macrophage and Clara cell preparations isolated from A/J mice 2 h following treatment with a single tumorigenic dose of 50 mg/kg AFB 1 ip ( n = 3, p < 0.05). Prior treatment with 300 kU/kg polyethylene glycol–conjugated catalase prevented the AFB 1 -induced increase in 8-OHdG levels in all mouse lung cell preparations ( n = 3, p < 0.05). These results support the possibility that oxidative DNA damage in mouse lung cells contributes to AFB 1 carcinogenicity.
Loading next page...

Preview Only. This article cannot be rented because we do not currently have permission from the publisher.

 
/lp/oxford-university-press/elevation-of-8-hydroxydeoxyguanosine-in-dna-from-isolated-mouse-lung-K1jd0810OU