Instant Access to the Journals you Need

Read Online + Print Articles, for just $40 a month.

Start Your Free Trial

Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage

Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage

Abstract

The peptide sequence N DKTH C was investigated as a site for efficient, specific cleavage of a fusion protein by cupric ions using a humanised ggr;1 Fab' as a model protein. The native upper hinge N DKTH C sequence was mutated to create a site resistant to cleavage by cupric ions and a N DKTH C sequence introduced between the hinge and a C-terminal FLAG peptide. Incubation of Fab' with Cu 2+ at 62°C at alkaline pHs resulted in removal of the FLAG peptide with efficiencies of up to 86%. Cleavage conditions did not detrimentally affect the Fab' protein. Use of the N DKTH C sequence along with cupric ions may provide a cost-effective method for large scale proteolytic cleavage of fusion proteins. Keywords : copper (II)/cupric/Fab'/FLAG peptide/protein cleavage
Loading next page...
 
/lp/oxford-university-press/efficient-site-specific-removal-of-a-c-terminal-flag-fusion-from-a-fab-uNZps8jkho

You're reading a free preview. Subscribe to read or print the entire article.

And millions more from thousands of peer-reviewed journals, for just $40/month.

Start Your Free Trial

What content is in DeepDyve?

  • Read and share from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more.
  • All the latest content is available, no embargo periods.

Rent Scholarly Articles?

  • Read the full article in your browser.
  • Each month you can print 20 pages for free.
  • Access all of your rentals from the cloud anywhere you have an internet connection.
  • Beautiful reading experience – Full charts and figures, just like the PDF.
  • Read as much as you'd like - whenever you'd like.

Happy Users

“In one word renting from DeepDyve is FANTASTIC!!! ... 99% of the time I only need access to an article for a month or so, so renting the articles is perfect for me.”

Adam S.

“Thanks for a great service! For an unaffiliated science blogger like myself this is like a dream come true.”

Seppo P.

“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”

Daniel C.

“Let me seize this opportunity and congratulate you on the service you are rendering to the scientific community.”

Joao B.