Dose rate effect on micronucleis induction in cytokinesis blocked human peripheral blood lymphocytes
Abstract
Abstract â The effect of dose rate on the induction of micronuclei (MN) in human peripheral blood lymphocytes was investigated over a range of dose rates from 0.125 Gy h 1 to 178.2 Gy h 1. The response of MN induction was ï¬tted with a linear quadratic model and the and coefï¬cients were estimated. It was found that values decrease with decreasing dose rate as in the case of chromosomal aberration. At the dose rate of 0.125 Gy h 1, pure linear response of MN induction was observed. An attempt was made to simulate the calibration curve for the purpose of biological dosimetry at different dose rates and exposure times. The yields when simulated with the exposure time or the dose rate are in agreement with experimental results. INTRODUCTION The dose rate effect of radiation is a biophysical phenomenon and is a consequence of the repair of sublethal damage. This effect is much less prominent for high LET radiations as compared to sparsely ionising radiations, such as gamma, X and beta rays. This is due to the production of more repairable sublethal damage with low LET radiation. Complex damage produced by densely ionising radiation, such as alpha rays,