Abstract Amyloid A (AA) amyloidosis is a leading cause of mortality in captive cheetahs ( Acinonyx jubatus ). We performed genome walking and PCR cloning and revealed that cheetahs have 4 SAA genes (provisionally named SAA1A , SAA1B , SAA3A , and SAA3B ). In addition, we identified multiple nucleotide polymorphisms in the 4 SAA genes by screening 51 cheetahs. The polymorphisms defined 4, 7, 6, and 4 alleles for SAA1A , SAA3A , SAA1B , and SAA3B , respectively. Pedigree analysis of the inheritance of genotypes for the SAA genes revealed that specific combinations of alleles for the 4 SAA genes cosegregated as a unit (haplotype) in pedigrees, indicating that the 4 genes were linked on the same chromosome. Notably, cheetah SAA1A and SAA1B were highly homologous in their nucleotide sequences. Likewise, SAA3A and SAA3B genes were homologous. These observations suggested a model for the evolution of the 4 SAA genes in cheetahs in which duplication of an ancestral SAA gene first gave rise to SAA1 and SAA3 . Subsequently, each gene duplicated one more time, uniquely making 4 genes in the cheetah genome. The monomorphism of the cheetah SAA1A protein might be one of the factors responsible for the high incidence of AA amyloidosis in this species.
Cheetahs Have 4 Serum Amyloid A Genes Evolved through Repeated Duplication Events
Abstract
Abstract Amyloid A (AA) amyloidosis is a leading cause of mortality in captive cheetahs ( Acinonyx jubatus ). We performed genome walking and PCR cloning and revealed that cheetahs have 4 SAA genes (provisionally named SAA1A , SAA1B , SAA3A , and SAA3B ). In addition, we identified multiple nucleotide polymorphisms in the 4 SAA genes by screening 51 cheetahs. The polymorphisms defined 4, 7, 6, and 4 alleles for SAA1A , SAA3A , SAA1B , and SAA3B , respectively. Pedigree analysis of the inheritance of genotypes for the SAA genes revealed that specific combinations of alleles for the 4 SAA genes cosegregated as a unit (haplotype) in pedigrees, indicating that the 4 genes were linked on the same chromosome. Notably, cheetah SAA1A and SAA1B were highly homologous in their nucleotide sequences. Likewise, SAA3A and SAA3B genes were homologous. These observations suggested a model for the evolution of the 4 SAA genes in cheetahs in which duplication of an ancestral SAA gene first gave rise to SAA1 and SAA3 . Subsequently, each gene duplicated one more time, uniquely making 4 genes in the cheetah genome. The monomorphism of the cheetah SAA1A protein might be one of the factors responsible for the high incidence of AA amyloidosis in this species.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Cheetahs Have 4 Serum Amyloid A Genes Evolved through Repeated Duplication Events
Chen, Lei; Une, Yumi; Higuchi, Keiichi; Mori, Masayuki
Follow Journal of Heredity
, Volume 103 (1): 115
Oxford University Press – Jan 1, 2012
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