Antistaphylococcal activities of CG400549, a new bacterial enoyl-acyl carrier protein reductase (FabI) inhibitor
Abstract
Abstract Objectives This study was performed to analyse in vitro and in vivo activities of CG400549, a new FabI inhibitor, against clinical isolates of staphylococci. The mode of action of CG400549 and resistance mechanism of Staphylococcus aureus against CG400549 were also investigated by genetic approaches. Methods In vitro activity of CG400549 was evaluated by the 2-fold agar sdilution method as described by the CLSI, and compared with those of oxacillin, erythromycin, ciprofloxacin, sparfloxacin, moxifloxacin, gemifloxacin, vancomycin, linezolid and quinupristin-dalfopristin. In vivo activity of CG400549 was determined against systemic infections in mice. Time–kill curves of CG400549 were analysed at concentrations of 1 × , 2 × and 4 × MIC against S. aureus strains. Results CG400549 had the lowest MICs among the test compounds against 238 clinical isolates of S . aureus (MIC 90 , 0.25 mg/L) and 51 clinical isolates of coagulase-negative staphylococci (MIC 90 , 1 mg/L). The activity of CG400549 was irrespective of whether the strains were methicillin-susceptible or -resistant. Furthermore, CG400549 was effective by oral or subcutaneous administration against systemic infections in mice. In a time–kill study, CG400549 at concentrations of 1 × MIC, 2 × MIC and 4 × MIC had a bacteriostatic activity during 24 h. A FabI-overexpressing S . aureus strain gave rise to an increase in the MIC of CG400549 compared with the parental strain, while the susceptibilities of the FabI-overexpressing S . aureus strain to the other antibacterial agents such as oxacillin, erythromycin and ciprofloxacin were not affected. This result showed that the mode of action of CG400549 was via inhibition of FabI, which is involved in biosynthesis of fatty acids in bacteria. Study of the resistance mechanism of S . aureus showed that CG400549-resistant mutants of S . aureus had an alteration in FabI at Phe-204 to Leu. Conclusions CG400549 had potent in vitro and in vivo activity against staphylococci, including methicillin-, ciprofloxacin- and multidrug-resistant staphylococci strains. This compound could be a good candidate for clinical development as a novel anti-MRSA drug. Keywords