A simple method for isolating hepatocytes from the liver with the aid of trypsin and their cultivation is described. The functional activity of the primary monolayer culture of hepatocytes was evaluated from the rate of albumin synthesis and secretion. The basal rate of albumin synthesis was fairly high during the first 10 minutes but after 2 hours decreased and reached plateau. On the contrary, the basal rate of albumin secretion rose with time and after 2 hours attained the rate of albumin synthesis. Human growth hormone (500 ng/ml) stimulated albumin output within 3 hours of incubation with hormonal stimulation in the cells from hypophysectomized rats being more pronounced as compared to that seen in hepatocytes from intact animals. A conclusion is made that the hepatocyte culture prepared by the method described can produce a release albumin and retains sensitivity to the hormonal regulator.
Procedure for producing a monolayer primary culture af rat hepatocytes and evaluation of its functional activity
Abstract
A simple method for isolating hepatocytes from the liver with the aid of trypsin and their cultivation is described. The functional activity of the primary monolayer culture of hepatocytes was evaluated from the rate of albumin synthesis and secretion. The basal rate of albumin synthesis was fairly high during the first 10 minutes but after 2 hours decreased and reached plateau. On the contrary, the basal rate of albumin secretion rose with time and after 2 hours attained the rate of albumin synthesis. Human growth hormone (500 ng/ml) stimulated albumin output within 3 hours of incubation with hormonal stimulation in the cells from hypophysectomized rats being more pronounced as compared to that seen in hepatocytes from intact animals. A conclusion is made that the hepatocyte culture prepared by the method described can produce a release albumin and retains sensitivity to the hormonal regulator.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Procedure for producing a monolayer primary culture af rat hepatocytes and evaluation of its functional activity
Pluzhnikova, G. N.; Ianakopulu, E. S.
Biulleten' eksperimental'noĭ biologii i meditsiny
MEDLINE Abstracts – Aug 1, 1981
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