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By means of autoradiography with thymidine-H3 the authors studied the mitotic cycle of a primary culture of the human testicle tumours on the 16th day of growth. Prolonged incubation with the isotope was employed. The following parameters of the mitotic cycle for the whole cellular population were established: T-83.6 hours, G + M = 60.25 hours, S = 5.35 hours, G2 = 18.0 hours. A conclusion was drawn that it was possible to use the primary culture to determine the mitotic cycle of human tumours.

Autoradiographic determination of the parameters of the mitotic cycle of tumors of human testis in tissue culture

Abstract

By means of autoradiography with thymidine-H3 the authors studied the mitotic cycle of a primary culture of the human testicle tumours on the 16th day of growth. Prolonged incubation with the isotope was employed. The following parameters of the mitotic cycle for the whole cellular population were established: T-83.6 hours, G + M = 60.25 hours, S = 5.35 hours, G2 = 18.0 hours. A conclusion was drawn that it was possible to use the primary culture to determine the mitotic cycle of human tumours.
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Autoradiographic determination of the parameters of the mitotic cycle of tumors of human testis in tissue culture

Terent'eva, T. G.; Sokolov, A. B.; Laskina, A. V.; Gibadulin, R. A.
Biulleten' eksperimental'noĭ biologii i meditsiny
MEDLINE Abstracts – Apr 1, 1975

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