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PCR Recipe: A Program for Setting Up PCR Reactions

PCR Recipe: A Program for Setting Up PCR Reactions


Biotech Software & Internet Report Volume 1, Number 6, 2000 PCR Recipe: A Program for Setting Up PCR Reactions Vinay K. Singh and Anil Kumar Bioinformatics Sub-Center, School of Biotechnology, Devi Ahilya University Introduction complete DNA-analysis and webbased software packages that design PCR primers and optimize reaction conditions. We have developed a software program for calculating how many different components of PCR mixture will need to be added to the master mix and then dispensed into the individual tubes. SVGA monitor, and a Windows 95/98/NT operating system. Copy protection for the program is provided by a software tool devised and developed by Ebot Technosoft Ltd. Indore, India (www.ebotsoft.com) The polymerase chain reaction (PCR) is a powerful molecular biology tool that has proven to be a sensitive and specific means for detecting minute quantities of nucleotide sequences. It is a technique sensitive enough to amplify DNA fragments over a billion-fold. The generation of amplified fragments (amplicons) by conventional PCR or modified methods of this technique is used extensively in biology, medicine, and forensic science. Researchers generally make a PCR master mix tube while setting up PCR for a large number of reactions, because dispensing all the components into individual
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