Monoclonal Antibodies Against hst-1 Gene Product
AbstractFour monoclonal antibodies (MAbs) against the hst-1 gene product (hst-1 protein) were obtained by a somatic cell hybridization technique. The recognition sites of these MAbs designated HS-131, HS-210, HS-233 and HS-276 on the hst-1 protein were evaluated by competitive binding assay with synthetic polypeptides. HS-131 MAb and HS-276 MAb recognize the epitope located within the 59-73 and the 197-206 amino acid sequences, respectively. The epitopes recognized by HS-210 and HS-233 MAbs could not be determined, but these MAbs showed neutralizing activity against hst-1 protein. Using HS-131 and HS-233 MAbs, a sensitive sandwich enzyme immunoassay (sandwich El A) has been developed. The assay sensitivity was 1.2-2.5 pg/well of hst-1 protein. Acidic and basic fibroblast growth factors were not crossreactive up to a concentration of 1 Î¼g/ml.