Gene Transfer and Living Release of Transforming Growth Factor-β3 for Cartilage Tissue Engineering Applications
Abstract
Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo . Herein, we constructed recombinant adenovirus encoding human transforming growth factor-β3 ( hTGF-β3 ) and practiced it in rat bone marrowâderived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-β3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-β3 gene has been successfully established and validated for cartilage tissue engineering applications.