Gene Transfer and Living Release of Transforming Growth Factor-Î²3 for Cartilage Tissue Engineering Applications
AbstractCartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo . Herein, we constructed recombinant adenovirus encoding human transforming growth factor-Î²3 ( hTGF-Î²3 ) and practiced it in rat bone marrowâderived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-Î²3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-Î²3 gene has been successfully established and validated for cartilage tissue engineering applications.