Subscribe to thousands of academic journals for just $40/month
Read and share the articles you need for your research, all in one place.

Evaluation of a Thin and Mechanically Stable Collagen Cell Carrier

Tissue Engineering, Part C: Methods , Volume 17 (12) – Dec 1, 2011


Mary Ann Liebert, Inc.
Copyright 2011, Mary Ann Liebert, Inc.
Publisher site
See Article on Publisher Site

Preview Only

Expand Tray Hide Tray

Evaluation of a Thin and Mechanically Stable Collagen Cell Carrier


The biological function of adherent cell populations strongly depends on the physical and biochemical properties of extracellular matrix molecules. Therefore, numerous biocompatible cell carriers have been developed to specifically influence cell attachment, proliferation, cellular differentiation, and tissue formation for diverse cell culture applications and cell-based therapies. In the present study, we evaluated the mechanical and the cell biological properties of a novel, thin, and planar collagen scaffold. The cell carrier is based on fibrillar bovine collagen type I and exhibits a low material thickness coupled with a high mechanical stability as measured by tensile tests. The influence of this new biomaterial on cell viability, proliferation, and cell differentiation was analyzed using 5-bromo-2-deoxyuridine (BrdU) proliferation assay, immunocytochemistry, water-soluble tetrazolium salt-1 assay (WST-1), live cell imaging, and electron microscopy. Cell culture experiments with the human osteosarcoma cell line Saos-2, human mesenchymal stem cells, and rodent cardiomyocytes demonstrated the in vitro biocompatibility of this chemically noncrosslinked scaffold. Both the mechanical characteristics and the in vitro biocompatibility of this collagen type I carrier facilitate the engineering of thin transferable tissue constructs and offer new possibilities in the fields of cell culture techniques, tissue engineering, and regenerative medicine.
Loading next page...

Preview Only. This article cannot be rented because we do not currently have permission from the publisher.