Access the full text.
Sign up today, get DeepDyve free for 14 days.
X. Wang, W. Vermeulen, J. Coursen, M. Gibson, Shawn Lupold, K. Forrester, G. Xu, L. Elmore, H. Yeh, J. Hoeijmakers, C. Harris (1996)
The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway.Genes & development, 10 10
C. Regan, M. Fuller (1988)
Interacting genes that affect microtubule function: the nc2 allele of the haywire locus fails to complement mutations in the testis-specific beta-tubulin gene of Drosophila.Genes & development, 2 1
C. Regan, M. Fuller (1990)
Interacting genes that affect microtubule function in Drosophila melanogaster: two classes of mutation revert the failure to complement between haync2 and mutations in tubulin genes.Genetics, 125 1
Paul Clute, J. Pines (1999)
Temporal and spatial control of cyclin B1 destruction in metaphaseNature Cell Biology, 1
J. Egly (2001)
The 14th Datta Lecture. TFIIH: from transcription to clinic.FEBS letters, 498 2-3
Sasha Akoulitchev, Danny Reinberg (1998)
The molecular mechanism of mitotic inhibition of TFIIH is mediated by phosphorylation of CDK7.Genes & development, 12 22
Matthew Wallenfang, G. Seydoux (2002)
cdk-7 is required for mRNA transcription and cell cycle progression in Caenorhabditis elegans embryosProceedings of the National Academy of Sciences of the United States of America, 99
Anne Keriel, A. Stary, A. Sarasin, C. Rochette-Egly, J. Egly (2002)
XPD Mutations Prevent TFIIH-Dependent Transactivation by Nuclear Receptors and Phosphorylation of RARαCell, 109
M. Martínez-Balbás, A. Dey, S. Rabindran, K. Ozato, Carl Wu (1995)
Displacement of sequence-specific transcription factors from mitotic chromatinCell, 83
Juhong Liu, Sasha Akoulitchev, A. Weber, H. Ge, S. Chuikov, D. Libutti, X. Wang, J. Conaway, C. Harris, R. Conaway, D. Reinberg, David Levens (2001)
Defective Interplay of Activators and Repressors with TFIIH in Xeroderma PigmentosumCell, 104
D. Hoogstraten, A. Nigg, H. Heath, L. Mullenders, R. Driel, J. Hoeijmakers, W. Vermeulen, A. Houtsmuller (2002)
Rapid switching of TFIIH between RNA polymerase I and II transcription and DNA repair in vivo.Molecular cell, 10 5
M. Araki, C. Masutani, Mitsuyo Takemura, Akio Uchida, K. Sugasawa, J. Kondoh, Y. Ohkuma, F. Hanaoka (2001)
Centrosome Protein Centrin 2/Caltractin 1 Is Part of the Xeroderma Pigmentosum Group C Complex That Initiates Global Genome Nucleotide Excision Repair*The Journal of Biological Chemistry, 276
C. Brown, W. Welch, S. Doxsey, E. White (1994)
Both viral (adenovirus E1B) and cellular (hsp 70, p53) components interact with centrosomesJournal of Cellular Physiology, 160
J. Long, A. Leresche, R. Kriwacki, J. Gottesfeld (1998)
Repression of TFIIH Transcriptional Activity and TFIIH-Associated cdk7 Kinase Activity at MitosisMolecular and Cellular Biology, 18
Jian Chen, S. Larochelle, Xiaoming Li, B. Suter (2003)
Xpd/Ercc2 regulates CAK activity and mitotic progressionNature, 424
A. Weber, Juhong Liu, I. Collins, D. Levens (2005)
TFIIH Operates through an Expanded Proximal Promoter To Fine-Tune c-myc ExpressionMolecular and Cellular Biology, 25
S. Iben, H. Tschochner, Mirko Bier, D. Hoogstraten, P. Hozák, J. Egly, I. Grummt (2002)
TFIIH Plays an Essential Role in RNA Polymerase I TranscriptionCell, 109
W. Vermeulen, E. Bergmann, J. Auriol, S. Rademakers, P. Frit, E. Appeldoorn, J. Hoeijmakers, J. Egly (2000)
Sublimiting concentration of TFIIH transcription/DNA repair factor causes TTD-A trichothiodystrophy disorderNature Genetics, 26
R. Thomas, M. Edwards, Ronald Marks (1996)
Translocation of the retinoblastoma gene product during mitosis.Experimental cell research, 223 2
H. Steeg, K. Kraemer (1999)
Xeroderma pigmentosum and the role of UV-induced DNA damage in skin cancer.Molecular medicine today, 5 2
S. Doxsey, D. McCollum, W. Theurkauf (2005)
Centrosomes in cellular regulation.Annual review of cell and developmental biology, 21
J. Gottesfeld, D. Forbes (1997)
Mitotic repression of the transcriptional machinery.Trends in biochemical sciences, 22 6
L. Mounkes, Richard Jones, B. Liang, W. Gelbart, M. Fuller (1992)
A Drosophila model for xeroderma pigmentosum and Cockayne's syndrome: haywire encodes the fly homolog of ERCC3, a human excision repair geneCell, 71
L. Hsu, R. White (1998)
BRCA1 is associated with the centrosome during mitosis.Proceedings of the National Academy of Sciences of the United States of America, 95 22
Background: The general transcription factor II H (TFIIH), comprised of a core complex and an associated CAK-complex, functions in transcription, DNA repair and cell cycle control. Mutations of the two largest subunits, p89 (XPB) and p80 (XPD), cause the hereditary cancer-prone syndrome xeroderma pigmentosum. Methods: The TFIIH subunit p89 was monitored during interphase and cell division by immunofluorescence staining, GFP-fusion constructs including deletions, live cell imaging and immuno-precipitations. Results: Here we demonstrate that during cell division, from prophase until telophase, the TFIIH core subunit p89, but not other subunits of TFIIH, associates with the centrosomes and the adjacent parts of the mitotic spindle. With overall constant levels throughout mitosis, p89 re-localizes to the newly formed nuclei by the end of mitosis. Furthermore, p89 interacts with the centrosomal protein γ-tubulin. Truncations of p89 result in an abnormal subcellular distribution during interphase and abolished centrosomal association during mitosis. Conclusions: Our observations suggest a so far unappreciated role for p89 in cell cycle regulation, and may be the structural basis for a long known, but hitherto unexplained interaction between p89 and tubulin.
Cellular Oncology – IOS Press
Published: Jan 1, 2010
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.