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Purification and crystallization of Escherichia coli pseudouridine synthase RluD

Purification and crystallization of Escherichia coli pseudouridine synthase RluD RluD is the pseudouridine () synthase responsible for forming 1911, 1915 and 1917 in Escherichia coli 23S RNA. Out of the 11 synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 A and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 A. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 A resolution. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section D: Biological Crystallography International Union of Crystallography

Purification and crystallization of Escherichia coli pseudouridine synthase RluD

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Purification and crystallization of Escherichia coli pseudouridine synthase RluD

Del Campo, M; Ofengand, J; Malhotra, A
Acta Crystallographica Section D: Biological Crystallography , Volume 59 (10): 1871 – Sep 19, 2003

Abstract

RluD is the pseudouridine () synthase responsible for forming 1911, 1915 and 1917 in Escherichia coli 23S RNA. Out of the 11 synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 A and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 A. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 A resolution.

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References (17)

Publisher
International Union of Crystallography
Copyright
Copyright (c) 2003 International Union of Crystallography
Subject
RluD, pseudouridine synthases
ISSN
0907-4449
eISSN
1399-0047
DOI
10.1107/S0907444903018468
Publisher site
See Article on Publisher Site

Abstract

RluD is the pseudouridine () synthase responsible for forming 1911, 1915 and 1917 in Escherichia coli 23S RNA. Out of the 11 synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 A and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 A. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 A resolution.

Journal

Acta Crystallographica Section D: Biological Crystallography – International Union of Crystallography

Published: Sep 19, 2003

Keywords: RluD ; pseudouridine synthases .

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