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A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to 2.1 A resolution

A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to... R88A, a mutant of the type IIE restriction endonuclease EcoRII, has been crystallized in space group P21, with unit-cell parameters a = 58.7, b = 92.4, c = 88.3 A, = 108.1Degrees. There are two monomers in the asymmetric unit and the solvent content is estimated to be 50% by volume. The crystals diffract to 2.1 A resolution, which is much higher than that of the wild type, which diffracted to 2.8 A resolution. The mutant crystals have been used in the identification of an excellent heavy-atom derivative. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section D: Biological Crystallography International Union of Crystallography

A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to 2.1 A resolution

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A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to 2.1 A resolution

Zhou, X.E; Wang, Y; Reuter, M; Mackeldanz, P; Kruger, D.H; Meehan, E.J; Chen, L
Acta Crystallographica Section D: Biological Crystallography , Volume 59 (5): 910 – Apr 25, 2003

Abstract

R88A, a mutant of the type IIE restriction endonuclease EcoRII, has been crystallized in space group P21, with unit-cell parameters a = 58.7, b = 92.4, c = 88.3 A, = 108.1Degrees. There are two monomers in the asymmetric unit and the solvent content is estimated to be 50% by volume. The crystals diffract to 2.1 A resolution, which is much higher than that of the wild type, which diffracted to 2.8 A resolution. The mutant crystals have been used in the identification of an excellent heavy-atom derivative.

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References (16)

Publisher
International Union of Crystallography
Copyright
Copyright (c) 2003 International Union of Crystallography
Subject
EcoRII, restriction endonucleases, type IIE, mutant
ISSN
0907-4449
eISSN
1399-0047
DOI
10.1107/S0907444903004086
Publisher site
See Article on Publisher Site

Abstract

R88A, a mutant of the type IIE restriction endonuclease EcoRII, has been crystallized in space group P21, with unit-cell parameters a = 58.7, b = 92.4, c = 88.3 A, = 108.1Degrees. There are two monomers in the asymmetric unit and the solvent content is estimated to be 50% by volume. The crystals diffract to 2.1 A resolution, which is much higher than that of the wild type, which diffracted to 2.8 A resolution. The mutant crystals have been used in the identification of an excellent heavy-atom derivative.

Journal

Acta Crystallographica Section D: Biological Crystallography – International Union of Crystallography

Published: Apr 25, 2003

Keywords: Eco RII; restriction endonucleases; type IIE; mutant.

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