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and cell death was measured by assessing glucose consumption, lactate production, release of lactate dehydrogenase, parathyroid hormone-related protein (PTHrP), tumour necrosis factor-alpha (TNF-α) and 8 ...
(H&E) staining, immunohistochemistry and flow cytometry. The latter was regarded as positive when >10% of cells reacted to pan- cytokeratin antibody MNF116. DNA ploidy was studied by propidium iodide ...
- cytokeratin (clones AE1/AE3; Leica), cytokeratin (CK) 14 (clone LL02; Leica), CD3 (clone LN10; Leica), and Ki-67 (clone MIB-1; Dako). Automated immunohistochemistry was performed using standard immunoperoxidase ...
background were used in this study. AGS cells were inoculated into the flank. The tumor volumes were calculated and growth curves were drawn. When the volume of the tumors reached 1000 mm3, the animals were ...
were used , with either PTUS or uninjured, as a control group. Cell ‐seeded stents were implanted into the rabbits strictured urethra. The histological and immunohistochemical findings were assessed after ...
and pancreatic intraepithelial neoplasia (PanIN). Methods and results: Reverse transcriptase‐polymerase chain reaction and Western blot were used to study TUBB3 expression in pancreatic cancer cell lines ...
and MethodsThis retrospective cohort study included all consecutive patients with pN0 gastric adenocarcinoma between January 2000 and December 2011 (n = 73). Immunohistochemistry using the pan- cytokeratin antibody ...
cell conditions. Cells were analyzed by real‐time PCR and fluorescent immunohistochemistry (FIHC). Tumor formation was assessed by injecting 1 × 106 CD133+CD49f+ cells intraperitoneally into immune ...
% sensitive, although TTF1 is easier to assess as a nuclear stain [33]. gMay be one or more lung masses. Abbreviations: CK, cytokeratin ; CUP, cancer of unknown primary; IHC, immunohistochemistry ; max, maximum ...
are precursors of subsequent distant relapse. Using monoclonal antibodies to epithelial cytokeratins or tumor-associated cell membrane glycoproteins, individual carcinoma cells can be detected on cytologic bone ...
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