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Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition

Details

Publisher
Informa Health
Copyright
© 2012 Informa Healthcare USA, Inc.
ISSN
1073-1199
eISSN
1532-4184
D.O.I.
10.3109/10731199.2012.657204
Publisher site
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Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition

Abstract

This study is related to the preparation of lysozyme-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyroptophan methylester) (nano-MIP) nanoparticles for purification of lysozyme. Nano-MIP particles were found to be 261 nm in diameter with a surface area of 1648 m 2 /g. According to the elemental analysis results, the particles contained 0.85 μmol MATrp/g polymer. The maximum lysozyme adsorption capacity was 1182.8 mg/g. Adsorbed lysozyme was desorbed with 94% recovery. It was observed that after five adsorption-desorption cycles there was no significant loss in adsorption capacity. In order to show the selectivity of the Lys-MIP nanoparticles, adsorption of lysozyme, bovine serum albumin (BSA), and cytochrome c were investigated.
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