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Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition

Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition

Abstract

This study is related to the preparation of lysozyme-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyroptophan methylester) (nano-MIP) nanoparticles for purification of lysozyme. Nano-MIP particles were found to be 261 nm in diameter with a surface area of 1648 m 2 /g. According to the elemental analysis results, the particles contained 0.85 μmol MATrp/g polymer. The maximum lysozyme adsorption capacity was 1182.8 mg/g. Adsorbed lysozyme was desorbed with 94% recovery. It was observed that after five adsorption-desorption cycles there was no significant loss in adsorption capacity. In order to show the selectivity of the Lys-MIP nanoparticles, adsorption of lysozyme, bovine serum albumin (BSA), and cytochrome c were investigated.
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