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tweezers under a dissecting microscope. The material was then further dried over silica gel for another 48 h, vacuum-sealed in plastic bags, stored at –20 °C for up to 3 months, and thawed over silica gel ...
at −20°C. Within one day of root collection, roots were rinsed with tap water to remove soil, blotted dry , and then dried in plastic bags with silica gel . After drying , the whole roots (containing ...
physical properties of the support that influence these parameters are the surface area and the pore size. The former should be high enough to allow high enzymatic loading within the internal structure ...
[29]. Other techniques have included a two-step method coupling an overnight incubation in 90% ethanol with subsequent silica -based desiccation [30], preservation using Whatman FTA cards [8,29,31 ...
then, the crude extract has been applied for working up and purification of its desired metabolites using a series of chromatographic techniques , starting with silica gel column chromatography and ended by Sephadex ...
gel plates ( silica Gel 60 F-254) using one of the following solvent systems: chloroform:methanol:water (65:25:4, v/v), chloroform:methanol:ammonia (65:30:4, v/v), petroleum ether:diethyl ether:acetic ...
bioavailability. The objective was therefore to formulate a stable amorphous solid dispersion of the drug with enhanced solubility and stability against enzymatic degradation. Methods Spray drying was used ...
on a Varian Gemini 200 MHz spectrometer. PhosphorImages were obtained using Molecular Dynamics PhosphorImager Model 400A. All chemicals were purchased from Aldrich Chemical Company. Silica gel for column ...
determines the efficacy of the enzymatic nucleic acid polymerization, the nucleic acid content or the individual concentrations of other magnesium-binding polyanions in the preparation will influence ...
) Analysis of enzymatic reaction products. Recombinant AtCPT7 was incubated with 14C-IPP together with either FPP or GGPP and the dephosphorylated reaction products were resolved on reverse-phase silica gel 60 ...
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