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with computational‐fluid‐dynamics analysis of flow in the homogenizer. Anionic SUVs are produced by the new method and incubated with poly‐l‐lysine (PLL)‐condensed plasmid DNA (pDNA) to generate complexes under ...
listeriolysin O (LLO) is a potentially promising agent. Methods LLO was incorporated in an anionic liposome ‐entrapped polycation‐condensed DNA delivery system (LPDII). Plasmid DNA was condensed using protamine ...
A novel downstream bioprocess was developed to obtain purified plasmid DNA (pDNA) from Escherichia coli ferments. The intermediate recovery and purification of the pDNA in cell lysate was conducted ...
oligodeoxynucleotide (ODN) or plasmid DNA coated by a neutral or anionic lipid bilayer. Moreover, our lipid vector demonstrates size stability and is able to retain a high content of enclosed plasmid DNA or antisense ...
encapsulation . Surface charge regulation (SCR) by electrolytes stabilizes liposome / plasmid DNA (pDNA) complexes by accelerated membrane fusion. Considering these facts, we hypothesized that SCR by electrolytes ...
is fundamentally different from other methods that have been used to form complexes of DNA associated with or encapsulated into zwitterionic or anionic liposomes . Most of the reported methods for assembling DNA ...
, such as electroporation,[3] viral genomes,[4,5] ballistic gold particles,[6] liposomal and polymeric nanoparticles and even direct injection of naked DNA .[7] Viral vectors have been observed to be highly efficient ...
. The most effective lipopolyamine- DNA complexes for gene transfer were those containing the shortest plasmid DNA . Introduction Viral vectors can mediate efficient gene transfer. However, three factors may ...
. By introducing exogenous genetic material, such as circular plasmid DNA containing a specific gene or short regulatory RNAs, into cells, target manipulations of gene expression or restoration of production ...
of cationic liposome - plasmid DNA complexes by polyamines and poly(ethylene glycol)-phospholipid conjugates for efficient in-vivo gene delivery . FEBS Lett . 400 : 233 – 237 . Google Scholar Crossref Search ADS ...
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