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for their effects on in vitro fertilization. Four antibodies decreased the fertilization rate from about 90% in controls to 8% (B5‐2 F7), 12% (A2‐2 A7), 13% (4‐G1), and 25% (A2‐2 F2), when the sperm cell ...
, and complementary DNA (cDNA) libraries were employed to PCR amplify human SAS1B and its transcripts. Monoclonal antibodies (mAbs) to human SAS1B were generated using mouse hybridomas. SAS1B deletion constructs were ...
Abstract We report an inhibitory effect of an anti -actin monoclonal antibody (mAb) on the human zona pellucida (ZP)-induced acrosome reaction (AR). Motile sperm were incubated with native human ZP ...
antibody , AS16, reacted with an 18 kDa sperm protein and seems to be a human homologue of the mouse monoclonal recombinant antisperm antibody that causes sperm agglutination. All these antibodies inhibited ...
membrane surface and studied its potential involvement in gamete membrane interaction during fertilisation. PARTICIPANTS/MATERIALS, SETTING, METHODS Monoclonal antibodies against human JUNO ( anti -hJUNO mAb ...
-propyl-β-CD concentration curves were analysed using an anti -β-tubulin monoclonal antibody (Figure 4A and B, lower panels). For the second experiment, mouse sperm were incubated in complete capacitation ...
-chemokine family and that it may play a role in brain inflammation processes. letters to nature 24. Okabe, M. et al. Effect of a monoclonal anti - mouse sperm antibody (OFB13) of interaction of mouse kit ...
mouse and human sperm . Although anti -Tssk3 antibodies recognized recombinant mouse and human Tssk3, this antibody did not detect Tssk3 in either mouse or human sperm , strongly suggesting that this kinase ...
with mAb to human and mouse ZP3, indicating that mAb bound to ZP3 do not affect embryonic development. Discussion The administration of monoclonal antibodies to human ZP3 results in highly effective , long ...
. Using in-vivo fertility and mouse / human sperm bindingin vitro , it should be possible to determine whether a single zona protein is sufficient to determine the specificity of sperm binding. If not, mouse ...
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