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of interest and background autofluorescence. We tested if the same principle can be exploited in bright field microscopy to bleach pigmented melanoma FFPE sections together with cell morphology maintenance ...
in protein concentration produces an increase in the number of signals rather than an increased staining intensity. With BlobFinder (for fluorescence readout) or BlobFinderBright (for bright - field readout ...
reflects the combination of green (direct fluorescence ) with red (immunofluorescence). Red cells are those detected only by immunofluorescence. (E): Another liver section was cleared of paraffin ...
mouse livers with CLSM. Fig. 5 View largeDownload slide 3D reconstruction of eosin- stained tissue images in a 100-μm section of normal mouse liver obtained by CLSM. (a) Bright - field light micrograph (eBL ...
distinct channels: blue (nuclei), green (placenta auto- fluorescence ), and red (co-localization between FV2 stain and SDC1). Scale bar represents 20 μm. (D) Bright - field visualization of placental tissue ...
://doi.org/10.1371/journal.pone.0284444.g001 To test the practical value of FCS, we performed histological stains by FCS, namely IF, HE and Sirius red in one single section (Fig 2A). The combination ...
is to enhance the productivity and quality of microscopy work by focusing on quick and cost-effective preparation of thick sections or plant specimen surfaces and efficient use of direct fluorescent stains . We ...
staining , as did S9 to some extent, and the fluorophores appeared to detect bacteria at the exclusion of DNA-containing plant organelles as gathered from bright - field and phase-contrast microscopy. The S9–PI ...
was performed. Second, attempts were made to combine staining of miR-21 with different cellular markers (CD3 and CD68).Third, fluorescence multiplexing by immunohistochemical staining of tissue for 3 different ...
tabDownload slide Sections stained with aniline blue fluorochrome. A, General view of a cross section of an incompatible interaction on SA27774 accession of M. truncatula observed under bright field . B ...
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